Dahlén G, Manji F, Baelum V, Fejerskov O
Department of Oral Microbiology, Faculty of Odontology, University of Göteborg, Sweden.
J Clin Periodontol. 1992 Jan;19(1):35-42. doi: 10.1111/j.1600-051x.1992.tb01146.x.
The aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural Kenyan population exhibiting poor oral hygiene and widespread loss of attachment (LA). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with LA greater than or equal to 4 mm; at least 5 mm LA and a pocket greater than or equal to 4 mm interproximally in a lower incisor ("test" site): and less than 2 mm LA and no pocket greater than or equal to 4 mm distal to a lower canine or mesial to a lower first premolar ("control" site). Age-matched "non-diseased" persons were identified on the basis of no sites with LA greater than 2 mm and no pockets greater than or equal to 4 mm associated with LA. Paperpoint samples from test and control sites as well as a scraping sample from the dorsum of tongue were examined for presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides intermedius, B. melaninogenicus group, Capnocytophaga, Selenomonas spp., and Wolinella recta. P. gingivalis was found in 79% of test sites and 36% of control sites in "diseased" persons, and in 18% and 35% of test and control sites, respectively, in "non-diseased" persons. "No other bacterial group discriminated significantly between test and control sites or between diseased and non-diseased subjects. The surprisingly high occurrence of P. gingivalis in non-diseased subjects, both subgingivally and on tongue, indicates that deep periodontal pockets are not prerequisite ecological environments for P. gingivalis establishment.
该研究的目的是评估来自肯尼亚农村成年人口中，口腔卫生差且普遍存在附着丧失（LA）的“患病”和“未患病”人群中，“测试”部位和“对照”部位某些假定的牙周病原体的发生率。14名年龄小于35岁的人，若至少有4个部位的附着丧失大于或等于4mm；下切牙邻面至少有5mm的附着丧失且牙周袋大于或等于4mm（“测试”部位）；下尖牙远中或下第一前磨牙近中附着丧失小于2mm且无大于或等于4mm的牙周袋（“对照”部位），则被归为“患病”类别。年龄匹配的“未患病”人群是根据无大于2mm附着丧失的部位以及无与附着丧失相关的大于或等于4mm的牙周袋来确定的。对测试部位和对照部位的纸尖样本以及舌背刮片样本进行检查，以检测伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、产黑色素普氏菌属、二氧化碳嗜纤维菌属、纤毛菌属和直肠沃廉菌的存在情况。在“患病”人群中，79%的测试部位和36%的对照部位发现了牙龈卟啉单胞菌；在“未患病”人群中，分别有18%和35%的测试部位和对照部位发现了该菌。没有其他细菌群在测试部位和对照部位之间，或在患病和未患病受试者之间有显著差异。牙龈卟啉单胞菌在未患病受试者的龈下和舌部出现的比例出奇地高，这表明深牙周袋并非牙龈卟啉单胞菌定植的必要生态环境。
