Pahl H L, Rosmarin A G, Tenen D G
Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
Blood. 1992 Feb 15;79(4):865-70.
The CD11b/CD18 heterodimeric surface antigen is expressed exclusively on human monocytes, macrophages, granulocytes, and natural killer cells. During differentiation of myeloid cell lines, CD11b steady state messenger RNA levels increase significantly; we show here that CD11b transcription rates increase commensurately. A 1.7-kb fragment of CD11b 5' flanking sequence directs expression of a reporter gene specifically in myeloid cell lines. Deletion analysis localizes elements directing high levels of tissue-specific reporter gene expression to the 412 bp proximal to the transcriptional start site. This sequence contains two consensus binding sites for Sp1, a GATA motif, and a purine-rich sequence that presents potential binding sites for members of the ets family of genes. Analysis of this promoter should result in the isolation of myeloid-specific transcription factors and the development of methods to direct the myeloid-specific expression of heterologous genes.
CD11b/CD18异二聚体表抗原仅在人类单核细胞、巨噬细胞、粒细胞和自然杀伤细胞上表达。在髓系细胞系分化过程中，CD11b稳态信使核糖核酸水平显著增加；我们在此表明CD11b转录率也相应增加。CD11b 5'侧翼序列的一个1.7kb片段指导报告基因在髓系细胞系中特异性表达。缺失分析将指导高水平组织特异性报告基因表达的元件定位到转录起始位点近端的412bp处。该序列包含两个Sp1共有结合位点、一个GATA基序和一个富含嘌呤的序列，该序列为ets基因家族成员提供了潜在的结合位点。对该启动子的分析应能分离出髓系特异性转录因子，并开发出指导异源基因髓系特异性表达的方法。
