利用同源裸鼠品系和原位杂交技术对nu基因进行定位。
Mapping of the nu gene using congenic nude strains and in situ hybridization.
作者信息
Takahashi Y, Shimizu A, Sakai T, Endo Y, Osawa N, Shisa H, Honjo T
机构信息
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
出版信息
J Exp Med. 1992 Mar 1;175(3):873-6. doi: 10.1084/jem.175.3.873.
The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome 11 by in situ hybridization using fluorescence-labeled cosmid probes. The results indicate the order of centromere-(41cM)-Il-3-(3cM)-Myhs- (4cM)-Acrb-(6cM)-Evi-2-(3cM)-Mpo-(5cM)- Hox-2. We have used congenic nude strains and examined which of the six DNA markers were derived from the original nude mouse. We found the Evi-2 locus is linked to the nu gene in all the informative, independent congenic nude strains. From these data, we could estimate the location of the nu gene, not only genetically but also physically within a region that spans approximately 17 megabases (9 cM) between the Acrb and Mpo genes.
利用位于小鼠11号染色体上的6个DNA标记（白细胞介素3 [Il-3]、Myhs、Acrb、Evi-2、Mpo和Hox-2）确定了导致无毛和无胸腺的nu基因的染色体定位。我们通过使用荧光标记的黏粒探针进行原位杂交，构建了11号染色体上这6个DNA标记的高分辨率物理图谱。结果表明着丝粒-(41厘摩)-Il-3-(3厘摩)-Myhs-(4厘摩)-Acrb-(6厘摩)-Evi-2-(3厘摩)-Mpo-(5厘摩)-Hox-2的顺序。我们使用了同源裸鼠品系，并检测了这6个DNA标记中哪些来自原始裸鼠。我们发现在所有信息丰富、独立的同源裸鼠品系中，Evi-2基因座与nu基因连锁。根据这些数据，我们不仅可以从遗传学上，还可以从物理上估计nu基因的位置，其位于Acrb和Mpo基因之间大约17兆碱基（9厘摩）的区域内。
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