Monaco F, Andreoli M, Cataudella S, Roche J
C R Seances Soc Biol Fil. 1976;170(1):59-64.
Thyroglobuline (Tg) biosynthesis has been studied in an adult fresh water lamprey Lampetra planeri (Bloch), by injection in coelomic cavity or by in vitro incorporation in follicles of branchial region of various radioactive labels: 125I-, 3H-leucine, 3H-mannose, 3H-galactose, N-acetyl mannosamine and 3H-N-acetylglucosamine. Labelling by all theses substances, present in Tg of mammals, proceeds efficiently in two hours. It has been demonstrated that the cyclostome studied synthetizes a 18 S iodinated glycoprotein analogous to Tg of higher vertebrates by its molecular size. It contains all the same hexose and N-acetyl derivatives of these in its sugar moiety and the same iodinated aminoacids. Purified 18 S Tg of the lamprey has been prepared; it is very poor in iodine (127I) and has a very low T3 and T4 content.
通过向成年淡水七鳃鳗（Lampetra planeri, Bloch）体腔注射或在体外将各种放射性标记物掺入鳃区滤泡的方法，对甲状腺球蛋白（Tg）的生物合成进行了研究，这些放射性标记物包括：125I、3H-亮氨酸、3H-甘露糖、3H-半乳糖、N-乙酰甘露糖胺和3H-N-乙酰葡糖胺。所有这些存在于哺乳动物Tg中的物质，在两小时内均能高效标记。已经证明，所研究的圆口纲动物合成了一种18S碘化糖蛋白，其分子大小与高等脊椎动物的Tg类似。其糖部分含有所有相同的己糖及其N-乙酰衍生物，以及相同的碘化氨基酸。已制备出纯化的七鳃鳗18S Tg；其碘（127I）含量极低，T3和T4含量也非常低。
