Fujinaga M, Maze M, Hoffman B B, Baden J M
Department of Anesthesia, Stanford University School of Medicine, California 94305.
Dev Biol. 1992 Apr;150(2):419-21. doi: 10.1016/0012-1606(92)90253-d.
Presomite stage rat embryos were cultured for 45-49 hr with medium containing various adrenergic agonists and antagonists. L-Norepinephrine but not D-norepinephrine (several orders of magnitude less potent than the L-isomer at alpha-1 adrenergic receptors) resulted in a dose-dependent increase of situs inversus similar to that found for phenylephrine, an alpha-1 adrenergic agonist. Prazosin, an alpha-1 adrenergic antagonist, inhibited phenylephrine-induced situs inversus in a dose-dependent manner. Neither dexmedetomidine, an alpha-2 adrenergic agonist, nor isoproterenol, a beta adrenergic agonist, caused situs inversus. These results provide pharmacological evidence that stimulation of alpha-1 but not of alpha-2 and beta adrenergic receptors modulates the control of left/right sidedness in rat embryos.
将原肠胚形成前阶段的大鼠胚胎在含有各种肾上腺素能激动剂和拮抗剂的培养基中培养45 - 49小时。L - 去甲肾上腺素而非D - 去甲肾上腺素（在α - 1肾上腺素能受体上的效力比L - 异构体低几个数量级）导致内脏反位呈剂量依赖性增加，类似于α - 1肾上腺素能激动剂去氧肾上腺素所导致的情况。α - 1肾上腺素能拮抗剂哌唑嗪以剂量依赖性方式抑制去氧肾上腺素诱导的内脏反位。α - 2肾上腺素能激动剂右美托咪定和β肾上腺素能激动剂异丙肾上腺素均未引起内脏反位。这些结果提供了药理学证据，表明刺激α - 1而非α - 2和β肾上腺素能受体可调节大鼠胚胎左右不对称性的控制。
