Hall D A
Connect Tissue Res. 1976;4(3):181-7. doi: 10.3109/03008207609152217.
The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the protein before reduction in Young's Modulus occurs. On the basis of these observations, it is confirmed that the enzyme/substrate complex in elastolysis is formed by interaction through a co-ordinately bound calcium atom and that this atom can be derived either from the substrate, where it stabilises the molecule, or from the enzyme.
研究了两种形式的弹性蛋白酶对弹性组织杨氏模量的影响。该酶的单体、贫钙形式在相当数量的蛋白质溶解之前会降低杨氏模量的值。另一方面，富含钙的聚合酶在杨氏模量降低之前会降解蛋白质。基于这些观察结果，证实了弹性蛋白分解过程中的酶/底物复合物是通过一个配位结合的钙原子相互作用形成的，并且这个原子可以来自底物（在底物中它稳定分子），也可以来自酶。
