Ohno S, Hora K, Furukawa T, Oguchi H
Department of Anatomy, Shinshu University School of Medicine, Matsumoto, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;61(5):351-8. doi: 10.1007/BF02890438.
In normal kidneys fixed by perfusion with tannic acid and glutaraldehyde, glomerular slit diaphragms have been reported to consist of highly ordered and isoporous substructures with a zipper-like configuration. We have re-evaluated the ultrastructure of fixed or unfixed glomeruli using quick-freezing and deep-etching (QF-DE) and freeze-substitution (QF-FS) methods. In the fixed slit diaphragms, zipper-like substructures were often observed by the QF-DE method. In contrast, in fresh unfixed glomeruli the slit diaphragms mainly consisted of non-porous substructures. The slit diaphragms were more widely opened in the fixed glomeruli, as examined by the QF-FS method. These results suggest that the foot processes shrink during tissue preparation by conventional methods with chemical fixatives, and that the broadening of slit diaphragms and zipper-like substructures are formed by the pulling apart of adjacent foot processes due to shrinkage.
在通过用鞣酸和戊二醛灌注固定的正常肾脏中，据报道肾小球裂孔隔膜由具有拉链状结构的高度有序且等孔的亚结构组成。我们使用快速冷冻和深度蚀刻（QF-DE）以及冷冻置换（QF-FS）方法重新评估了固定或未固定肾小球的超微结构。在固定的裂孔隔膜中，通过QF-DE方法经常观察到拉链状亚结构。相比之下，在新鲜未固定的肾小球中，裂孔隔膜主要由无孔亚结构组成。通过QF-FS方法检查发现，固定肾小球中的裂孔隔膜开口更宽。这些结果表明，在使用化学固定剂的传统方法进行组织制备过程中，足突会收缩，并且裂孔隔膜的变宽和拉链状亚结构是由于收缩导致相邻足突拉开而形成的。
