Shimomura S, Hisamatsu K, Fujii Y, Ohno S
Department of Otorhinolaryngology, Yamanashi Medical University, Japan.
J Anat. 1996 Jun;188 ( Pt 3)(Pt 3):651-9.
In order to clarify the natural ultrastructure of goblet cells in the rat nasal mucosa, they were examined by the quick-freezing and freeze-substitution (QF-FS) or deep-etching (QF-DE) methods for comparison with conventional fixation methods. Some nasal mucosal tissues were unstimulated; others were stimulated with acetylcholine or substance P. The QF-FS method yielded fewer artefacts on transmission electron microscopy than conventional fixation methods. In the stimulated goblet cells, most of the secretory granules appeared to be loose in the matrix and more distorted in shape. By the QF-DE method, they were observed 3-dimensionally to be larger in size and aggregated together. In contrast, the secretory granules in the unstimulated goblet cells were mostly round and small, and separate from each other. It is concluded that the ultrastructure of secretory granules is artefactually modified by conventional fixation methods and that granule structure in goblet cells alters during the secretory process.
为了阐明大鼠鼻黏膜杯状细胞的天然超微结构，对其采用快速冷冻和冷冻置换（QF-FS）或深度蚀刻（QF-DE）方法进行检查，以便与传统固定方法进行比较。一些鼻黏膜组织未受刺激；其他组织则用乙酰胆碱或P物质刺激。在透射电子显微镜下，QF-FS方法产生的伪像比传统固定方法少。在受刺激的杯状细胞中，大多数分泌颗粒在基质中似乎松散，形状更扭曲。通过QF-DE方法，三维观察到它们尺寸更大且聚集在一起。相比之下，未受刺激的杯状细胞中的分泌颗粒大多呈圆形且较小，并且彼此分开。得出的结论是，传统固定方法会人为改变分泌颗粒的超微结构，并且杯状细胞中的颗粒结构在分泌过程中会发生变化。
