Vacca A, Frassanito A, Rimoldi D, Dammacco F, Carrel S
Department of Biomedical Sciences and Human Oncology, University of Bari Medical School, Italy.
Anticancer Res. 1992 Jan-Feb;12(1):1-9.
The reactivity of four monoclonal antibodies (MAbs) directed against IFN-gamma inducible antigens with melanocytic cells was investigated in the course of local and systemic tumor progression of human malignant melanoma. Frozen sections of histologically defined melanocytic tissues at different stages of progression were stained with these MAbs using an indirect immunoperoxidase technique. The reactivity of MAbs Me15/B3 and Me15/F9, directed against two different epitopes of a 90-kDa molecule, was found to correlate with melanoma progression. Indeed, a significantly lower percentage of small than of advanced primary melanomas or metastases stained positively. A differential staining of nevocytic and dysplastic nevi was further observed for these two MAbs, which were also non-reactive with normal skin melanocytes. The reactivity of MAb Me14/D12, which identifies the intercellular adhesion molecule ICAM-1 and MAb Mel14/F12, directed against a 40-kDa molecule, was found to be independent of the Breslow thickness of primary melanomas. Both the latter MAbs stained a high proportion of nevocytic and dysplastic nevi. The co-expression of the surface molecules defined by MAbs Me14/D12, Me15/B3 and Me15/F9 in the course of melanoma progression was also analyzed. The frequency of this co-expression increased according to the Breslow thickness of primary melanomas. In addition, up to 100% of metastases, as opposed to 20% of dysplastic nevi, were found to be simultaneously stained by these three MAbs. It is therefore conceivable that high-risk melanocytic lesions might be identified by the use of a combination of MAbs directed against IFN-gamma regulated antigens.
在人类恶性黑色素瘤的局部和全身肿瘤进展过程中，研究了四种针对干扰素-γ诱导抗原的单克隆抗体（MAb）与黑素细胞的反应性。使用间接免疫过氧化物酶技术，用这些单克隆抗体对组织学定义的不同进展阶段黑素细胞组织的冰冻切片进行染色。发现针对90 kDa分子的两个不同表位的单克隆抗体Me15/B3和Me15/F9的反应性与黑色素瘤进展相关。实际上，原发性小黑色素瘤染色阳性的比例明显低于晚期原发性黑色素瘤或转移灶。进一步观察到这两种单克隆抗体对痣细胞痣和发育异常痣的染色存在差异，它们也与正常皮肤黑素细胞无反应。发现识别细胞间粘附分子ICAM-1的单克隆抗体Me14/D12和针对40 kDa分子的单克隆抗体Mel14/F12的反应性与原发性黑色素瘤的 Breslow厚度无关。后两种单克隆抗体均对高比例的痣细胞痣和发育异常痣进行染色。还分析了在黑色素瘤进展过程中由单克隆抗体Me14/D12、Me15/B3和Me15/F9定义的表面分子的共表达情况。这种共表达的频率根据原发性黑色素瘤的 Breslow厚度而增加。此外，发现高达100%的转移灶，与20%的发育异常痣相反，可被这三种单克隆抗体同时染色。因此，可以设想，通过使用针对干扰素-γ调节抗原的单克隆抗体组合，可能识别出高危黑素细胞病变。
