Kondo K, Umemoto A, Akimoto S, Uyama T, Hayashi K, Ohnishi Y, Monden Y
Second Department of Surgery, School of Medicine, University of Tokushima, Japan.
Biochem Biophys Res Commun. 1992 Mar 31;183(3):1139-46. doi: 10.1016/s0006-291x(05)80309-2.
The reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis and sequencing were used to examine p53 gene alterations in 18 surgical specimens of primary lung cancers obtained in Japan. Somatic mutations resulting in amino acid changes were found in eight of the 18 cases (44%). Seven missense mutations were located in amino acid-conserved domains or their vicinities (codons 110 to 307). Most mutations were found at G-C pairs, suggesting that specific carcinogens are involved in the etiology of lung cancer. The p53 mutations showed a significant association with a history of smoking (P = 0.0294). We suggest that the p53 mutations may be associated with smoking-induced lung carcinogenesis.
采用逆转录-聚合酶链反应-单链构象多态性（RT-PCR-SSCP）分析和测序方法，对在日本获取的18例原发性肺癌手术标本中的p53基因改变进行检测。18例病例中有8例（44%）发现了导致氨基酸改变的体细胞突变。7个错义突变位于氨基酸保守结构域或其附近（密码子110至307）。大多数突变发生在G-C碱基对处，提示特定致癌物参与肺癌的病因学。p53突变与吸烟史显著相关（P = 0.0294）。我们认为p53突变可能与吸烟诱导的肺癌发生有关。
