Schena M, Davis R W
Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305-5307.
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3894-8. doi: 10.1073/pnas.89.9.3894.
Homeobox genes encode a large family of homeodomain proteins in animal systems. To test whether such genes are also abundant in higher plants, degenerate oligonucleotides complementary to sequences encoding the recognition helix (helix three) of the homeodomain were used to screen genomic and cDNA libraries from the plant Arabidopsis thaliana. Analysis of 8 of the 41 cDNAs isolated revealed that each encodes a presumptive homeodomain; interestingly, most of these clones also contain a leucine zipper motif tightly linked to the homeodomain. It is concluded that Arabidopsis encodes a large family of homeodomain proteins, including members that contain a homeodomain/leucine-zipper (HD-Zip) motif.
同源异型框基因在动物系统中编码一大类同源异型结构域蛋白。为了检测此类基因在高等植物中是否也很丰富，与编码同源异型结构域识别螺旋（螺旋三）的序列互补的简并寡核苷酸被用于筛选来自植物拟南芥的基因组文库和cDNA文库。对分离得到的41个cDNA中的8个进行分析发现，每个都编码一个假定的同源异型结构域；有趣的是，这些克隆中的大多数还包含一个与同源异型结构域紧密相连的亮氨酸拉链基序。得出的结论是，拟南芥编码一大类同源异型结构域蛋白，包括含有同源异型结构域/亮氨酸拉链（HD-Zip）基序的成员。
