Dervan P A, Magee H M, Buckley C, Carney D N
Department of Pathology, Mater Hospital, Dublin, Ireland.
Am J Clin Pathol. 1992 May;97(5 Suppl 1):S21-8.
Cell proliferation can be studied by a variety of techniques. However, most require fresh tissue. To evaluate cell proliferation in formalin-fixed paraffin-embedded sections, the authors immunohistochemically studied 35 tumors and 11 samples of normal/hyperplastic tissue with PC10, a monoclonal antibody directed against proliferating cell nuclear antigen. Results were compared with those obtained with Ki-67 on fresh tissues. There was no significant difference between proliferating cell nuclear antigen and Ki-67 counts, which were strongly correlated (r = 0.8). Proliferating cell nuclear antigen positivity was easier to evaluate because morphology was better preserved in formalin-fixed tissue. The authors conclude that PC10 is an alternative to Ki-67 in evaluating cell proliferation and has the advantage of reacting with formalin-fixed paraffin-embedded tissue.
细胞增殖可以通过多种技术进行研究。然而，大多数技术都需要新鲜组织。为了评估福尔马林固定石蜡包埋切片中的细胞增殖情况，作者使用PC10（一种针对增殖细胞核抗原的单克隆抗体）对35个肿瘤以及11个正常/增生组织样本进行了免疫组织化学研究。将结果与在新鲜组织上使用Ki-67获得的结果进行了比较。增殖细胞核抗原和Ki-67计数之间没有显著差异，二者高度相关（r = 0.8）。增殖细胞核抗原阳性更容易评估，因为在福尔马林固定的组织中形态保存得更好。作者得出结论，在评估细胞增殖方面，PC10是Ki-67的一种替代方法，并且具有与福尔马林固定石蜡包埋组织反应的优势。
