免疫金标记是一种定量方法，这已在对微绒毛膜囊泡中的氨肽酶N的研究中得到证实。
Immunogold labelling is a quantitative method as demonstrated by studies on aminopeptidase N in microvillar membrane vesicles.
作者信息
Hansen G H, Wetterberg L L, Sjöström H, Norén O
机构信息
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
出版信息
Histochem J. 1992 Mar;24(3):132-6. doi: 10.1007/BF01047462.
Microvillar membrane vesicle preparations with varying content of aminopeptidase N were prepared from enterocytes of the pig small intestine. Postembedding immunogold labelling of aminopeptidase N was performed on these glutaraldehyde/paraformaldehyde-fixed, osmium tetroxide-treated and Epon-embedded microvillar membrane vesicles. The number of gold particles per micrometre microvillar membrane (labelling intensity) was calculated and compared to the corresponding enzymatic activity. A very close relationship was found between labelling intensity and aminopeptidase N activity, demonstrating that postembedding immunogold labelling can be used quantitatively.
从猪小肠肠细胞制备了具有不同氨肽酶N含量的微绒毛膜囊泡制剂。对这些经戊二醛/多聚甲醛固定、四氧化锇处理并包埋在环氧树脂中的微绒毛膜囊泡进行了氨肽酶N的包埋后免疫金标记。计算每微米微绒毛膜的金颗粒数量（标记强度），并与相应的酶活性进行比较。发现标记强度与氨肽酶N活性之间存在非常密切的关系，表明包埋后免疫金标记可用于定量分析。
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