Moncharmont P, Bonnard M, Bernaud J, Rigal D, Mellier G, Monier J C
CRTS-Lyon, Beynost.
J Gynecol Obstet Biol Reprod (Paris). 1992;21(2):214-8.
During pregnancy, the fetus is an hemiallograft perfectly tolerated by the mother. With the aim of elucidating the mechanism of this tolerance, we have looked to see whether lymphocyte subsets are modified by pregnancy. Using monoclonal antibodies and flow cytometry, we have studied the changes in the immune profile of normal pregnant women and compared them with non pregnant women. In pregnant women, a significant decrease in the percentage of CD3+ cells is observed in the first trimester (61.1 +/- 14.7% versus 73.8 +/- 6.8%; p less than 0.001). The same data are obtained for CD4+ cells (38.2 +/- 10.7% versus 44.0 +/- 7.0%; p less than 0.05) and CD8+ cells (22.8 +/- 5.6% versus 28.0 +/- 8.9%; p less than 0.05). On the other hand, B lymphocytes (CD19+), monocytes (Leu M3+) and natural killer (NK) cells (Leu7+) remain stable during pregnancy. CD11a+ cells decreased during the 1st and 2nd trimesters. Lastly, activated T lymphocytes (CD3+DR+, CD8+DR+) are not modified. Using absolute numbers, a significant decrease is shown only for CD3+ cells in the 2nd and 3rd trimesters and for CD11a+ cells in the 2nd trimester of pregnancy. The decrease of T lymphocyte subsets, NK and CD11a+ cells during pregnancy partially explains the tolerance for the fetus.
在孕期，胎儿是一种能被母亲完美耐受的半同种异体移植物。为了阐明这种耐受机制，我们研究了孕期淋巴细胞亚群是否发生改变。我们使用单克隆抗体和流式细胞术，研究了正常孕妇免疫谱的变化，并将其与非孕妇进行比较。在孕妇中，孕早期CD3⁺细胞百分比显著下降（61.1±14.7%对73.8±6.8%；p<0.001）。CD4⁺细胞（38.2±10.7%对44.0±7.0%；p<0.05）和CD8⁺细胞（22.8±5.6%对28.0±8.9%；p<0.05）也得到相同数据。另一方面，B淋巴细胞（CD19⁺）、单核细胞（Leu M3⁺）和自然杀伤（NK）细胞（Leu7⁺）在孕期保持稳定。CD11a⁺细胞在孕1期和孕2期减少。最后，活化T淋巴细胞（CD3⁺DR⁺、CD8⁺DR⁺）未发生改变。采用绝对计数时，仅显示孕2期和孕3期CD3⁺细胞以及孕2期CD11a⁺细胞有显著减少。孕期T淋巴细胞亚群、NK细胞和CD11a⁺细胞的减少部分解释了对胎儿的耐受性。
