Saccani Jotti G, Fontanesi M, Bombardieri E, Gabrielli M, Veronesi P, Bianchi M, Becchi G, Bogni A, Tardini A
Institute of Pathology, University of Parma, School of Medicine, Italy.
Int J Biol Markers. 1992 Jan-Mar;7(1):35-42.
The expression of oncogene products related to cell growth (c-erbB-2, c-myc, ras p21, EGFR) was investigated in benign (15 cases) and malignant breast lesions (20 cases) by means of immunohistochemistry using the avidin-biotin-peroxidase technique with polyclonal and monoclonal antibodies. The aim of this study was to evaluate the relationship between the staining positivity and various morphological and biological features, such as tumour type, grading, hormone receptor status and cell kinetic parameters. In benign breast lesions, as expected, the kinetic parameters were low, both for Ki-67 and LI. All the specimens showed a diploid condition (the DI being equal to 1) and we found a limited degree of immunoreactivity for all the growth factors and oncogene products. In breast cancer we studied the distribution of immunohistochemical positivity for EGFR, c-erbB-2, c-myc, ras p21 and Ki-67, which was related to age, nodal status, ER and PgR receptor status, LI, DI and histopathological grading. A significant positive correlation was found both between ras p21 expression and nodal status and ER-ICA positivity. We observed a strong correlation between LI and Ki-67 and an inverse relation between Ki-67 and ER expression. These findings suggest the importance of studying the relationship between prognostic factors which may provide preoperative prediction in the biological behaviour of breast cancer, not only on biopsy specimens, but also on fine needle aspirates.
采用抗生物素蛋白-生物素-过氧化物酶技术，使用多克隆和单克隆抗体，通过免疫组织化学方法研究了15例良性和20例恶性乳腺病变中与细胞生长相关的癌基因产物（c-erbB-2、c-myc、ras p21、表皮生长因子受体）的表达情况。本研究的目的是评估染色阳性与各种形态学和生物学特征之间的关系，如肿瘤类型、分级、激素受体状态和细胞动力学参数。正如预期的那样，在良性乳腺病变中，Ki-67和LI的动力学参数均较低。所有标本均显示二倍体状态（DNA指数等于1），并且我们发现所有生长因子和癌基因产物的免疫反应性程度有限。在乳腺癌中，我们研究了表皮生长因子受体、c-erbB-2、c-myc、ras p21和Ki-67免疫组化阳性的分布情况，其与年龄、淋巴结状态、雌激素受体和孕激素受体状态、LI、DNA指数和组织病理学分级有关。在ras p21表达与淋巴结状态和雌激素受体免疫细胞化学阳性之间均发现显著的正相关。我们观察到LI和Ki-67之间有很强的相关性，以及Ki-67与雌激素受体表达之间呈负相关。这些发现表明，研究预后因素之间的关系很重要，这不仅可以在活检标本上，也可以在细针穿刺抽吸物上对乳腺癌的生物学行为进行术前预测。
