Gossrau R, Richter W
Department of Anatomy, Free University of Berlin, Germany.
Acta Histochem Suppl. 1992;42:39-49.
The frequent use of methods different from enzyme histochemistry evokes the question, whether it is still useful to apply methods of catalytic enzyme histochemistry to study the nervous system. In this brief overview it is shown, that catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function. This was enabled by methodological progress, i.e., the modification of already existing procedures or the development of new techniques for the visualization and measurement of enzymes in tissue sections using their catalytic properties. The methods for acetylcholinesterase, monoamine oxidase as well as certain exoglycosidases and phosphatases will be given as examples. The application of these procedures as well as of methods for proteases, oxyradical-generating oxidases and enzymes involved in the metabolism of amino acid and other transmitters will illustrate, that qualitative (localization) and quantitative (measurement) catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function.
频繁使用不同于酶组织化学的方法引发了一个问题，即应用催化酶组织化学方法来研究神经系统是否仍然有用。在这篇简短的综述中表明，催化酶组织化学仍可有助于更好地理解神经系统功能。这得益于方法学的进步，即对现有程序的改进或开发利用组织切片中酶的催化特性进行可视化和测量的新技术。将以乙酰胆碱酯酶、单胺氧化酶以及某些外切糖苷酶和磷酸酶的方法为例。这些程序以及蛋白酶、产氧自由基氧化酶和参与氨基酸及其他递质代谢的酶的方法的应用将说明，定性（定位）和定量（测量）催化酶组织化学仍可有助于更好地理解神经系统功能。
