Amano K, Suzuki N, Hatakeyama H, Kasahara Y, Fujii S, Fukushi K, Suto T, Mahara F
Central Research Laboratory, University School of Medicine, Japan.
Acta Virol. 1992 Jan;36(1):67-72.
Of the sera which were positive to Rickettsia tsutsugamushi by indirect immunoperoxidase test, approximately 80% sera were positive to a Proteus OXK antigen by Weil-Felix test at 10 or more days after the onset of fever, while only 10% sera were positive within 9 days from the onset of fever. In ELISA using the OXK antigen, almost all of the paired sera of tsutsugamushi disease (TD) patients increased on the IgM antibody titres with the rise of their titres by Weil-Felix test, whereas the IgG antibody titres of these sera were unrelated with the titres of Weil-Felix test. We suspect that the reactivity of TD patients sera to the OXK antigen in Weil-Felix test was derived from the reactivity of the IgM antibody against the OXK antigen common with R. tsutsugamushi. The patient sera infected with a Japanese isolate of spotted fever group rickettsia (SFGR) cross-reacted with the Thai Tick Typhus (TTT) strain of SFGR by indirect immunoperoxidase test. In Weil-Felix test, the reactivity of these sera to OX2 antigen were higher than that to OX19 antigen, like the sera infected with other SFGR, except of R. rickettsii. These sera also reacted with TTT and OX2 antigens by ELISA. The titres of IgM antibody against OX2 antigen in the sera in ELISA were in parallel with the titres of the sera against OX2 antigen in Weil-Felix test, but not the titres of IgG antibody. We suggest that the reactivity of the patient sera infected with SFGR to OX2 antigen of Weil-Felix test is dependent on the IgM antibody.
通过间接免疫过氧化物酶试验对恙虫病东方体呈阳性反应的血清中，约80%的血清在发热开始10天或更久后进行外斐试验时对变形杆菌OXK抗原呈阳性反应，而在发热开始9天内只有10%的血清呈阳性反应。在使用OXK抗原的ELISA试验中，恙虫病（TD）患者几乎所有配对血清的IgM抗体滴度随外斐试验滴度升高而升高，而这些血清的IgG抗体滴度与外斐试验滴度无关。我们怀疑TD患者血清在外斐试验中对OXK抗原的反应性源自针对与恙虫病东方体共同的OXK抗原的IgM抗体的反应性。感染日本斑点热群立克次体（SFGR）分离株的患者血清通过间接免疫过氧化物酶试验与SFGR的泰国蜱传斑疹伤寒（TTT）菌株发生交叉反应。在外斐试验中，这些血清对OX2抗原的反应性高于对OX19抗原的反应性，如同感染除立氏立克次体之外的其他SFGR的血清一样。这些血清在ELISA试验中也与TTT和OX2抗原发生反应。ELISA试验中血清针对OX2抗原的IgM抗体滴度与外斐试验中血清针对OX2抗原的滴度平行，但与IgG抗体滴度无关。我们认为感染SFGR的患者血清在外斐试验中对OX2抗原的反应性取决于IgM抗体。
