Amano K, Suzuki N, Fujita M, Nakamura Y, Suto T
Central Research Laboratory, Akita University School of Medicine, Japan.
Microbiol Immunol. 1993;37(12):927-33. doi: 10.1111/j.1348-0421.1993.tb01726.x.
Sera from 17 patients of scrub typhus in the acute and convalescent phases were tested by indirect immunoperoxidase test, Weil-Felix (WF) test, enzyme-linked immunosorbent assay (ELISA), and immunoblotting. In the comparison of antibody titers between acute- and convalescent-phase sera, we recognized a parallelism of increment between the titers in WF test and titers of immunoglobulin M (IgM) in ELISA against Proteus mirabilis strain OXK-whole cells and OXK-lipopolysaccharides (Proteus OXK-LPS). Furthermore, IgM antibodies from almost all of WF test-positive sera recognized LPS from Proteus OXK in immunoblotting. Based on these results, it was concluded that IgM antibody rather than IgG may participate in WF test, and that Proteus OXK-LPS may have one of antigenic epitopes common to the components of R. tsutsugamushi.
采用间接免疫过氧化物酶试验、外斐（WF）试验、酶联免疫吸附测定（ELISA）和免疫印迹法对17例恙虫病患者急性期和恢复期血清进行检测。在比较急性期和恢复期血清抗体滴度时，我们发现WF试验滴度与ELISA中针对奇异变形杆菌OXK全细胞和OXK脂多糖（变形杆菌OXK-LPS）的免疫球蛋白M（IgM）滴度之间存在平行升高。此外，几乎所有WF试验阳性血清中的IgM抗体在免疫印迹中都能识别变形杆菌OXK的LPS。基于这些结果，得出结论：可能是IgM抗体而非IgG参与WF试验，并且变形杆菌OXK-LPS可能具有恙虫病东方体成分共有的一个抗原表位。
