Shi D, He G, Cao S, Pan W, Zhang H Z, Yu D, Hung M C
Department of Pathology, Cancer Hospital/Cancer Institute, Shanghai Medical University, People's Republic of China.
Mol Carcinog. 1992;5(3):213-8. doi: 10.1002/mc.2940050308.
The c-erbB-2/neu gene encodes a transmembrane protein of 185 kDa (p185) with tyrosine kinase activity and extensive sequence homology to epidermal growth factor receptor. Amplification and overexpression of the c-erbB-2/neu gene has been shown in certain human tumors and is postulated to be important in human carcinogenesis. High levels of expression of the c-erbB-2/neu gene have been reported in non-small-cell lung cancer (NSCLC) cell lines and primary tumors from the United States. Since geographical and cultural factors may contribute to the development of certain types of cancer, we examined p185 examined p185 expression in 120 tumors from Chinese patients with lung cancers of different cell types and used immunohistochemical staining to determine the extent and general significance of p185 expression in human primary lung cancer. Our results demonstrate that 58.8% of the NSCLCs expressed p185 and that expression of p185 was observed only in NSCLC and not in small-cell lung cancers. Thirty-three of 41 adenocarcinomas and 24 of 55 squamous cell carcinomas among the NSCLCs examined were found to express p185 at levels different from those of normal lung. For the squamous cell carcinomas, p185 expression was correlated with lymph node metastasis (P less than 0.01), but for the adenocarcinomas, it was not (P greater than 0.05). In addition, expression of p185 in NSCLC was significantly more frequent in patients in advanced clinical stages. Our findings indicate that p185 expression is a frequent event and a general phenomenon in NSCLC and is correlated with poor clinical prognostic indicators, suggesting that expression of p185 may be of potential prognostic importance in NSCLC.
c-erbB-2/neu基因编码一种185 kDa的跨膜蛋白（p185），具有酪氨酸激酶活性，与表皮生长因子受体有广泛的序列同源性。c-erbB-2/neu基因的扩增和过表达已在某些人类肿瘤中得到证实，并被认为在人类致癌过程中起重要作用。在美国的非小细胞肺癌（NSCLC）细胞系和原发性肿瘤中，已报道c-erbB-2/neu基因有高水平表达。由于地理和文化因素可能导致某些类型癌症的发生，我们检测了120例来自中国不同细胞类型肺癌患者肿瘤中的p185表达，并采用免疫组化染色来确定p185表达在人类原发性肺癌中的程度和普遍意义。我们的结果表明，58.8%的NSCLC表达p185，且仅在NSCLC中观察到p185表达，而在小细胞肺癌中未观察到。在所检测的NSCLC中，41例腺癌中有33例、55例鳞状细胞癌中有24例被发现表达的p185水平与正常肺组织不同。对于鳞状细胞癌，p185表达与淋巴结转移相关（P<0.01），但对于腺癌则不然（P>0.05）。此外，NSCLC中p185的表达在临床晚期患者中明显更常见。我们的研究结果表明，p185表达在NSCLC中是常见事件和普遍现象，并且与不良临床预后指标相关，提示p185表达在NSCLC中可能具有潜在的预后重要性。
