在大鼠表皮中鉴定与小鼠Thy-1⁺树突状表皮细胞等效的细胞。

Identification of an equivalent to murine Thy-1+ dendritic epidermal cells in the rat epidermis.

作者信息

Shimada S, Shibagaki N, Tanaka T, Kubota Y, Hashimoto Y, Tamaki K

机构信息

Department of Dermatology, Yamanashi Medical College, Sendai, Japan.

出版信息

J Dermatol Sci. 1992 Jan;3(1):68-71. doi: 10.1016/0923-1811(92)90010-9.

DOI:10.1016/0923-1811(92)90010-9

PMID:1350463

Abstract

In the murine epidermis, there exist Thy-1+ dendritic epidermal cells (Thy-1+DEC). These cells are Thy-1+, CD45+, CD3+ and asialo GM1+ but CD5-, CD4-, CD8-, or Ia-1-, and express T cell receptor (TCR) gamma delta. Recently, most of these TCR gamma delta of Thy-1 DEC are shown to consist of a V gamma 3-V delta 1 combination. There has been no evidence that the same type of cell population exists in other species except mice. In this study, we investigated the existence of a Thy-1+DEC equivalent in the rat epidermis. The epidermal sheets obtained from rats were stained with various monoclonal antibodies to rat lymphocytes. We developed a monoclonal antibody (1F4) to rat CD3 complex. 1F4 stained thymocytes and peripheral T cells and also immunoprecipitated T cell receptor with CD3 complex. Using 1F4 and a recently developed monoclonal antibody to rat TCR alpha beta, we could identify dendritic CD4-, CD8-, CD5-, CD3+, TCR alpha beta- cells in the rat epidermis. These CD3+, TCR alpha beta- cells are strong candidates as an equivalent to TCR gamma delta + murine Thy-1+ DEC.

摘要

在小鼠表皮中，存在Thy-1⁺树突状表皮细胞（Thy-1⁺DEC）。这些细胞Thy-1⁺、CD45⁺、CD3⁺且无唾液酸GM1⁺，但CD5⁻、CD4⁻、CD8⁻或Ia-1⁻，并表达T细胞受体（TCR）γδ。最近发现，这些Thy-1 DEC的大多数TCRγδ由Vγ3-Vδ1组合构成。除小鼠外，尚无证据表明其他物种存在相同类型的细胞群体。在本研究中，我们调查了大鼠表皮中是否存在等效的Thy-1⁺DEC。从大鼠获取的表皮片用多种针对大鼠淋巴细胞的单克隆抗体进行染色。我们研制了一种针对大鼠CD3复合物的单克隆抗体（1F4）。1F4可对胸腺细胞和外周T细胞进行染色，还能与CD3复合物一起免疫沉淀T细胞受体。利用1F4和最近研制的一种针对大鼠TCRαβ的单克隆抗体，我们能够在大鼠表皮中识别出树突状CD4⁻、CD8⁻、CD5⁻、CD3⁺、TCRαβ⁻细胞。这些CD3⁺、TCRαβ⁻细胞极有可能等同于TCRγδ⁺小鼠Thy-1⁺DEC。