Jarrell J F, Sevcik M, Stuart G
Department of Obstetrics and Gynaecology, Foothills Hospital, University of Calgary, Alberta, Canada.
Reprod Toxicol. 1992;6(2):133-5. doi: 10.1016/0890-6238(92)90114-9.
Glutathione (GSH) is an important intracellular thiol capable of altering metabolism following exposure to certain important biologic toxicants including radiation and cyclophosphamide. In order to evaluate the inhibition of glutathione synthesis in the ovary, 30-day-old Sprague Dawley rats were treated with either saline or 0.6 mumol/kg (0.133 mg/kg), 6.0 mumol/kg (1.33 mg/kg), or 4.5 mmol/kg (1000 mg/kg) buthionine sulphoximine (BSO) IP and sacrificed at 0, 1, 2, 4, 6, 8, and 24 h. There was an inhibition of glutathione synthesis with 4.5 mmol/kg (1000 mg/kg) BSO with a nadir at 8 h (P less than 0.001) and complete recovery at 24 h. In the subsequent experiments rats were divided into four groups. All animals received either saline or BSO 4.5 mmol/kg/day (1000 mg/kg/day) from day 27 to 30 of life and either saline or PMSG 5 IU IP on day 29 of life. BSO reduced ovarian content of GSH (saline-saline compared with BSO-saline, P less than 0.0001), which was countered by the prior administration of PMSG (BSO-saline compared with BSO-PMSG, P less than 0.005). Glutathione levels were as follows: saline-saline 4.3 +/- 0.04; saline-PMSG 5.0 +/- 0.4; BSO-saline 2.13 +/- 0.2; BSO-PMSG 3.24 +/- 0.2 nmol/mg ovary. These findings suggest the ovary is susceptible to GSH depletion by in vivo administration of BSO. Gonadotropin (PMSG) is capable of effecting a partial return of total ovarian GSH content.
谷胱甘肽（GSH）是一种重要的细胞内硫醇，在暴露于某些重要的生物毒素（包括辐射和环磷酰胺）后能够改变新陈代谢。为了评估卵巢中谷胱甘肽合成的抑制情况，对30日龄的斯普拉格-道利大鼠腹腔注射生理盐水或0.6 μmol/kg（0.133 mg/kg）、6.0 μmol/kg（1.33 mg/kg）或4.5 mmol/kg（1000 mg/kg）的丁硫氨酸亚砜胺（BSO），并在0、1、2、4、6、8和24小时处死。4.5 mmol/kg（1000 mg/kg）的BSO抑制了谷胱甘肽的合成，在8小时时达到最低点（P<0.001），并在24小时时完全恢复。在随后的实验中，将大鼠分为四组。所有动物在出生后第27天至30天接受生理盐水或4.5 mmol/kg/天（1000 mg/kg/天）的BSO，在出生后第29天腹腔注射生理盐水或5 IU的孕马血清促性腺激素（PMSG）。BSO降低了卵巢中GSH的含量（生理盐水-生理盐水组与BSO-生理盐水组相比，P<0.0001），而预先注射PMSG可对抗这种情况（BSO-生理盐水组与BSO-PMSG组相比，P<0.005）。谷胱甘肽水平如下：生理盐水-生理盐水组4.3±0.04；生理盐水-PMSG组5.0±0.4；BSO-生理盐水组2.13±0.2；BSO-PMSG组3.24±0.2 nmol/mg卵巢。这些发现表明，通过体内给予BSO，卵巢易受GSH耗竭的影响。促性腺激素（PMSG）能够使卵巢中总GSH含量部分恢复。
