关于人类对豚草过敏原蒿属植物V（Ra5S）和三裂叶豚草V（Ra5G）反应中涉及的HLA II类等位基因的研究。

Studies of the HLA class II alleles involved in human responses to ragweed allergens Ambrosia artemisiifolia V (Ra5S) and Ambrosia trifida V (Ra5G).

作者信息

Goldstein R, Yang W H, Drouin M A, Karsh J

机构信息

Division of Rheumatology, Ottawa General Hospital, Canada.

出版信息

Tissue Antigens. 1992 Mar;39(3):122-7. doi: 10.1111/j.1399-0039.1992.tb01921.x.

DOI:10.1111/j.1399-0039.1992.tb01921.x

PMID:1350871

Abstract

Previous studies have associated skin test sensitivity and specific IgE response to Ambrosia artemisiifolia V (Amb a V) with HLA-DR2, and to Ambrosia trifida V (Amb t V) with HLA-DRw52 haplotypes in atopic individuals. Using HLA class II typing by restriction fragment length polymorphism (RFLP) analysis with DRB, DQB and DQA DNA probes to define the HLA-D alleles, we have demonstrated the association of the DQw6 in 16 out of 16 (100%) Amb a V-responsive individuals, compared to 3 out of 18 (17%) ragweed-sensitive but Amb a V-nonresponsive individuals (p = 5.7 x 10(-6), RR greater than 75). We suggest that the DQw6 association with Amb a V sensitivity may be a reflection of an association with the DQA*0102 allele. This suggests an association of a particular HLA class II allele with an immune response to a well-characterized antigen (Amb a V). The HLA-DRw52 haplotypes in the Amb t V-sensitive individuals are not of one particular subtype. The HLA-DRw52 association with Amb t V sensitivity may reside in homologous DRB1 alleles linked on HLA-DRw52-bearing haplotypes.

摘要

先前的研究已将特应性个体中对豚草五聚体变应原（Amb a V）的皮肤试验敏感性和特异性IgE反应与HLA - DR2相关联，而将对三裂叶豚草五聚体变应原（Amb t V）的反应与HLA - DRw52单倍型相关联。通过使用限制片段长度多态性（RFLP）分析，采用DRB、DQB和DQA DNA探针进行HLA II类分型来确定HLA - D等位基因，我们发现16名（100%）对Amb a V有反应的个体中有16名存在DQw6，相比之下，18名对豚草敏感但对Amb a V无反应的个体中有3名存在DQw6（p = 5.7×10⁻⁶，相对风险大于75）。我们认为DQw6与Amb a V敏感性的关联可能反映了与DQA*0102等位基因的关联。这表明特定的HLA II类等位基因与对一种特征明确的抗原（Amb a V）的免疫反应相关。对Amb t V敏感的个体中的HLA - DRw52单倍型并非某一特定亚型。HLA - DRw52与Amb t V敏感性的关联可能存在于与携带HLA - DRw52的单倍型相连的同源DRB1等位基因中。