Hazenberg M P, de Visser H
Department of Immunology, Erasmus University Rotterdam, The Netherlands.
Eur J Clin Chem Clin Biochem. 1992 Mar;30(3):141-4.
A method is reported for the determination of N-acetylmuramyl-L-alanine amidase in serum. Muramic acid, released from the interpeptide bridges of Brevibacterium divaricatum peptidoglycan, is measured by a modified colorimetric method. Using this procedure, it was possible to determine N-acetylmuramyl-L-alanine amidase in aliquots of less than 10 microliters human serum with an incubation time of 10 min. Amidase activity was found in all the sera tested (n = 11). The relevance of this simple and fast assay is discussed.
