Maden M, Horton C, Graham A, Leonard L, Pizzey J, Siegenthaler G, Lumsden A, Eriksson U
Developmental Biology Research Centre, King's College London, U.K.
Mech Dev. 1992 Mar;37(1-2):13-23. doi: 10.1016/0925-4773(92)90011-8.
We describe here the distribution of cellular retinoic acid-binding protein I (CRABP I) in the head of the early mouse embryo from day 8 to day 13 of gestation, using both in situ hybridisation to localise mRNA and immunocytochemistry to localise protein. The distribution of mRNA and protein was found to be identical. CRABP I first appeared in part of the presumptive hindbrain of the presomite embryo and then became localised to rhombomeres 2, 4, 5 and 6. The only other area of expression in the cephalic neuroepithelium was in a part of the midbrain roof. The neural crest and its mesenchymal derivatives, the branchial arches, expressed CRABP I and crest could be seen streaming from the neuroepithelium of individual rhombomeres into particular branchial arches. This suggested a fate map could be constructed describing the rhombomeric origin of branchial arch mesenchyme. Later in development, axons throughout the hindbrain expressed CRABP I. The results are considered in terms of the role of retinoic acid in the specification of neuronal phenotype in the hindbrain and in axon outgrowth.
我们在此描述妊娠第8天至第13天早期小鼠胚胎头部细胞视黄酸结合蛋白I（CRABP I）的分布情况，采用原位杂交定位mRNA以及免疫细胞化学定位蛋白的方法。发现mRNA和蛋白的分布是相同的。CRABP I首先出现在原肠胚前脑的部分假定后脑区域，然后定位于菱脑节2、4、5和6。在头部神经上皮中唯一的其他表达区域是中脑顶的一部分。神经嵴及其间充质衍生物鳃弓表达CRABP I，并且可以看到神经嵴从各个菱脑节的神经上皮流入特定的鳃弓。这表明可以构建一个命运图谱来描述鳃弓间充质的菱脑节起源。在发育后期，整个后脑的轴突都表达CRABP I。从视黄酸在后脑神经元表型特化和轴突生长中的作用方面对结果进行了讨论。
