Sved A F, Backes M G
Department of Behavioral Neuroscience, University of Pittsburgh, Pennsylvania 15260.
J Auton Nerv Syst. 1992 May 15;38(3):219-29. doi: 10.1016/0165-1838(92)90033-d.
The ability of vagal and glossopharyngeal afferent neurons to retrogradely transport 3H-D-aspartate from the nucleus tractus solitarius to the nodose and petrosal ganglia was examined. Injections of 3H-D-aspartate centered in the medial NTS at the rostral-caudal level of the area postrema failed to consistently label cells in the nodose and petrosal ganglia. In 5 of the 10 rats studied no retrogradely labeled neurons were observed in these ganglia ipsilateral to the injection site, while in the other 5 rats a small number of cells (less than 3%) were labeled. Injections of 3H-D-aspartate into the NTS consistently produced retrograde labeling of neurons in the ipsilateral paratrigeminal area. In addition, many heavily labeled neurons were observed in the injected as well as the contralateral NTS. Injections of 3H-D-asparate into the spinal trigeminal nucleus consistently labeled neurons in the trigeminal ganglion. Since the uptake and retrograde transport of 3H-D-aspartate appears to be characteristic of neurons that use glutamate or aspartate as a neurotransmitter, these results suggest that vagal and glossopharyngeal afferents are not glutamatergic or aspartatergic.
研究了迷走神经和舌咽神经传入神经元从孤束核向结节神经节和岩神经节逆行转运³H-D-天冬氨酸的能力。在最后区头-尾水平的内侧孤束核注射³H-D-天冬氨酸，未能始终如一地标记结节神经节和岩神经节中的细胞。在所研究的10只大鼠中，有5只在注射部位同侧的这些神经节中未观察到逆行标记的神经元，而在另外5只大鼠中，有少量细胞（少于3%）被标记。向孤束核注射³H-D-天冬氨酸始终会使同侧三叉旁区的神经元产生逆行标记。此外，在注射侧以及对侧孤束核中观察到许多标记强烈的神经元。向三叉神经脊束核注射³H-天冬氨酸会始终标记三叉神经节中的神经元。由于³H-D-天冬氨酸的摄取和逆行转运似乎是使用谷氨酸或天冬氨酸作为神经递质的神经元的特征，这些结果表明迷走神经和舌咽神经传入纤维不是谷氨酸能或天冬氨酸能的。
