Berger T, Walz W, Schnitzer J, Kettenmann H
Department of Neurobiology, University of Heidelberg, Germany.
J Neurosci Res. 1992 Jan;31(1):21-7. doi: 10.1002/jnr.490310104.
Whole-cell transmitter-activated currents were recorded with the patch-clamp technique from glial cells in thin frontal brain slices of the corpus callosum. In slices from 6- to 8-day-old mice, glioblasts were predominantly found, while oligodendrocytes were predominant in slices from 10- to 13-day-old mice. These developmental stages could be readily distinguished by their K+ channel pattern and their morphology and ultrastructural features. Both cell types expressed GABA and glutamate receptors in this in situ preparation. GABA responses showed similarities to those described for GABAA receptors, i.e., they were mimicked by muscimol, blocked by bicuculline, and enhanced by pentobarbital. Glutamate responses showed similarities to those of the kainate/quisqualate receptor subtype. The amplitude of GABA-activated currents recorded in oligodendrocytes was significantly smaller than that from glioblasts, while glutamate responses did not show marked differences in either cell type.
采用膜片钳技术，在胼胝体额叶脑薄片中的神经胶质细胞上记录全细胞递质激活电流。在6至8日龄小鼠的脑片中，主要发现的是成神经胶质细胞，而在10至13日龄小鼠的脑片中，少突胶质细胞占主导。这些发育阶段可以通过它们的钾离子通道模式、形态以及超微结构特征轻易区分。在这种原位标本中，两种细胞类型均表达GABA和谷氨酸受体。GABA反应与针对GABAA受体所描述的反应相似，即它们可被蝇蕈醇模拟，被荷包牡丹碱阻断，并被戊巴比妥增强。谷氨酸反应与海人藻酸/quisqualate受体亚型的反应相似。在少突胶质细胞中记录到的GABA激活电流的幅度显著小于成神经胶质细胞中的，而谷氨酸反应在两种细胞类型中均未显示出明显差异。
