To K Y, Lai Y K, Feng T Y, Chen C C
Department of Botany, National Taiwan University, Taipei, Republic of China.
Genome. 1992 Apr;35(2):220-4. doi: 10.1139/g92-033.
Chloroplast DNA isolated from wild-type Nicotiana plumbaginifolia and 12 maternally inherited streptomycin-resistant mutants was digested with various restriction enzymes and the resultant patterns were compared. No gross structural alterations of the chloroplast genome were detected in any mutants; however, variant patterns owing to the gain or loss of a restriction site were found in three mutants, SR1007, SR1019, and SR1022. The variant patterns in SR1019 and SR1022 are identical and are the results of mutation in the psbG gene coding for a chloroplast membrane protein G, and that in SR1007 is due to mutation in the 16S rRNA gene. Inheritance of the variant patterns in mutants SR1007 and SR1019 was studied. The results showed that the variant patterns and streptomycin resistance were co-transmitted in reciprocal crosses.
从野生型白花烟草和12个母系遗传的抗链霉素突变体中分离出叶绿体DNA，用各种限制性内切酶进行消化，并比较所得图谱。在任何突变体中均未检测到叶绿体基因组的总体结构改变；然而，在三个突变体SR1007、SR1019和SR1022中发现了由于限制性酶切位点的获得或缺失而产生的变异图谱。SR1019和SR1022中的变异图谱相同，是编码叶绿体膜蛋白G的psbG基因突变的结果，而SR1007中的变异图谱则是由于16S rRNA基因突变所致。对突变体SR1007和SR1019中变异图谱的遗传进行了研究。结果表明，在正反交中，变异图谱和链霉素抗性是共同传递的。
