To K Y, Liu C I, Liu S T, Chang Y S
Department of Microbiology and Immunology, Chang-Gung Medical College, Taoyuan, Taiwan, Republic of China.
Plant J. 1993 Jan;3(1):183-6. doi: 10.1046/j.1365-313x.1993.t01-10-00999.x.
Single-stranded conformation polymorphism (SSCP) analysis was used to examine the mutations of the chloroplast 16S rRNA locus of streptomycin-resistant mutants in Nicotiana plumbaginifolia. DNA fragments of 121, 517, 968 and 1578 bp, each possessing a known point mutation, were generated by polymerase chain reaction (PCR). The resulting fragments were denatured and separated by nondenaturing polyacrylamide gel electrophoresis. Compared to the patterns of the wild-type DNA fragments, the bands of the single-stranded DNA fragments of 121 and 517 bp with base changes were shifted. However, no pattern variations were detected from the DNA fragments of 968 and 1578 bp generated from both wild-type and mutants.
采用单链构象多态性（SSCP）分析来检测烟草中链霉素抗性突变体叶绿体16S rRNA基因座的突变。通过聚合酶链反应（PCR）产生了分别具有已知点突变的121、517、968和1578 bp的DNA片段。将所得片段变性后，通过非变性聚丙烯酰胺凝胶电泳进行分离。与野生型DNA片段的条带模式相比，具有碱基变化的121和517 bp单链DNA片段的条带发生了迁移。然而，野生型和突变体产生的968和1578 bp的DNA片段未检测到条带模式变化。
