Nkhumeleni F S, Raubenheimer E J, Dauth J, van Heerden W F, Smith P D, Pitout M J
Department of Oral Pathology and Biology, University of Pretoria, Republic of South Africa.
Arch Oral Biol. 1992 Feb;37(2):157-8. doi: 10.1016/0003-9969(92)90012-w.
Dentine of permanent mandibular incisors from nine individuals was hydrolysed and the amino acid composition determined by ion-exchange chromatography against a standard calibrant of 41 amino acids. Nineteen amino acids were detected, including small quantities of 1-methylhistidine and asparagine, two amino acids whose existence had apparently not been recorded before in human dentine. The total content of hydroxylysine plus lysine varied between 2.6 and 3.3 residues per 100 (SD, 0.74) in different teeth, which therefore did not support previous studies that had proposed a constant total value. This and other quantifiable differences between present and previous findings may be the result of the different methods and the influence of dietary and other regional factors on dentinogenesis.
对9名个体的恒下颌切牙牙本质进行水解，并通过离子交换色谱法针对41种氨基酸的标准校准物测定氨基酸组成。检测到19种氨基酸，包括少量的1-甲基组氨酸和天冬酰胺，这两种氨基酸在人牙本质中的存在此前显然未被记录。不同牙齿中羟赖氨酸加赖氨酸的总含量在每100个残基2.6至3.3之间变化（标准差为0.74），因此不支持先前提出恒定总值的研究。目前和先前研究结果之间的这种以及其他可量化差异可能是不同方法以及饮食和其他区域因素对牙本质形成的影响所致。
