Kuijpers T W, Hoogerwerf M, Kuijpers K C, Schwartz B R, Harlan J M
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Immunol. 1992 Aug 1;149(3):998-1003.
Normal human neutrophils bound an as yet unclustered mAb designated BS-1. The Ag immunoprecipitated with BS-1 was blotted by CD43 mAb (and vice versa), and is therefore identical to the large sialoglycoprotein. The CD43 Ag expression on the neutrophil surface is decreased upon neutrophil activation with the chemoattractant FMLP or with PMA. This can be (at least partially) explained by the release of CD43+ material with an altered electrophoretic mobility into the extracellular medium of the neutrophils upon activation. Cross-linking of the CD43 Ag with BS-1 also invoked neutrophil activation by itself: F(ab)2 fragments of BS-1-induced neutrophil aggregation, in contrast to F(ab) fragments. Neither respiratory burst activity nor a significant rise in intracellular Ca2+ level or actin polymerization were observed. The transient neutrophil aggregation response was largely CD18 dependent, especially in the initial phase of homotypic clustering. However, a significant CD18-independent mechanism contributed thereafter to the neutrophil aggregation, as was further substantiated by the use of cultured T (and EBV-transformed B) cell clones of a patient with a leukocyte adhesion deficiency. CD43 is the first molecule described on neutrophils able to induce adhesive properties in a dual fashion.
正常人类中性粒细胞可结合一种尚未聚集的单克隆抗体（mAb），命名为BS-1。与BS-1发生免疫沉淀的抗原可被CD43单克隆抗体印迹（反之亦然），因此与大唾液酸糖蛋白相同。在用趋化因子FMLP或佛波酯（PMA）激活中性粒细胞后，其表面的CD43抗原表达会降低。这至少可以部分解释为，激活后电泳迁移率改变的CD43+物质释放到中性粒细胞的细胞外介质中。CD43抗原与BS-1交联本身也会引发中性粒细胞激活：与F(ab)片段相比，BS-1的F(ab)2片段可诱导中性粒细胞聚集。未观察到呼吸爆发活性、细胞内Ca2+水平显著升高或肌动蛋白聚合。短暂的中性粒细胞聚集反应很大程度上依赖于CD18，尤其是在同型聚集的初始阶段。然而，随后一种不依赖CD18的重要机制对中性粒细胞聚集起作用，这一点通过对一名白细胞黏附缺陷患者的培养T（和EB病毒转化的B）细胞克隆的研究得到了进一步证实。CD43是在中性粒细胞上描述的第一个能够以双重方式诱导黏附特性的分子。
