Fischer G F, Pickl W F, Faé I, Ebner C, Ferreira F, Breiteneder H, Vikoukal E, Scheiner O, Kraft D
Institute for Blood Group Serology (National Blood Group Reference Laboratory), WHO, Vienna, Austria.
Hum Immunol. 1992 Apr;33(4):259-65. doi: 10.1016/0198-8859(92)90333-i.
The association of the human IgE response against Bet v I, the major allergen of birch pollen, and the HLA-DR and DQ phenotype was studied. Birch pollen allergic patients showed a typical case history, positive skin-prick test, and positive RAST with birch pollen extracts. They were divided into two groups. Group I (n = 37) consisted of individuals generating IgE antibodies that selectively reacted with Bet v I. Their serum IgE did not react with minor allergens from birch pollen as tested by immunoblot analysis, nor did they show a response against allergens from a panel of grass and other tree pollen or perennial allergens from animals and fungi as determined by skin-prick test. Patients belonging to group II (n = 34) possessed IgE reacting with Bet v I plus one or more additional allergens. The control group consisted of 637 healthy blood donors. Comparison of the frequencies of RFLP-defined HLA-DR and DQ alleles in patients and the control group revealed that the distribution of DRB3 alleles in group I patients differed significantly from that in the control group: A higher frequency of the DRw52a/c alleles in comparison to the control group (pcorr less than 0.02) was observed. In addition, alleles defined by nucleotide sequences coding for the amino acid sequence tyrosine-phenylalanine-histidine at positions 30-32 of the beta chain of DR molecules were found with a higher frequency in patient group I (pcorr less than 0.02), too. These alleles comprise DRw52a/c and some DRB1 alleles.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了人类针对桦树花粉主要过敏原Bet v I的IgE反应与HLA - DR和DQ表型之间的关联。桦树花粉过敏患者具有典型的病史、皮肤点刺试验阳性以及桦树花粉提取物RAST阳性。他们被分为两组。第一组（n = 37）由产生与Bet v I选择性反应的IgE抗体的个体组成。通过免疫印迹分析检测，他们的血清IgE与桦树花粉中的次要过敏原不反应，通过皮肤点刺试验确定，他们对一组草花粉、其他树花粉或动物和真菌的多年生过敏原也无反应。属于第二组（n = 34）的患者的IgE与Bet v I以及一种或多种其他过敏原反应。对照组由637名健康献血者组成。对患者和对照组中RFLP定义的HLA - DR和DQ等位基因频率进行比较，结果显示第一组患者中DRB3等位基因的分布与对照组有显著差异：与对照组相比，DRw52a/c等位基因频率更高（校正p值小于0.02）。此外，在患者第一组中也发现，编码DR分子β链第30 - 32位氨基酸序列酪氨酸 - 苯丙氨酸 - 组氨酸的核苷酸序列所定义的等位基因频率更高（校正p值小于0.02）。这些等位基因包括DRw52a/c和一些DRB1等位基因。（摘要截短于250字）
