Verdon R, Mirelman D, Sansonetti P J
Unité de Pathogénie microbienne moléculaire, Institut Pasteur, Paris.
Res Microbiol. 1992 Jan;143(1):67-74. doi: 10.1016/0923-2508(92)90035-m.
The establishment of a relationship between Entamoeba histolytica and certain bacteria may contribute to the expression and/or enhancement of the pathogenicity of this parasite. Recent experiments have shown that bacteria expressing mannose-binding lectins on their surface could attach to mannose-containing molecules on the surface of amoebae. In this study, we established a model of interaction between. E. histolytica and Shigella flexneri. Using well-characterized mutants of S. flexneri, we studied the role of type I pili expression and the invasive phenotype of S. flexneri in the interaction between amoebae and the bacteria. Type I pili expression allowed attachment and subsequent internalization of S. flexneri by amoebae, these events were not observed in isogenic strains that did not express type I pili. Invasive as well as non-invasive variants of S. flexneri expressing type I pili were slowly digested by amoebae following internalization. Morphological studies showed that the specific features of the interaction depend on the dynamics of the distribution of mannose residues on the amoebic membrane during the interaction.
溶组织内阿米巴与某些细菌之间关系的建立可能有助于该寄生虫致病性的表达和/或增强。最近的实验表明，表面表达甘露糖结合凝集素的细菌能够附着在变形虫表面含甘露糖的分子上。在本研究中，我们建立了溶组织内阿米巴与福氏志贺菌之间的相互作用模型。利用福氏志贺菌特征明确的突变体，我们研究了I型菌毛表达及福氏志贺菌的侵袭表型在变形虫与细菌相互作用中的作用。I型菌毛的表达使福氏志贺菌能够被变形虫附着并随后内化，在不表达I型菌毛的同基因菌株中未观察到这些现象。表达I型菌毛的福氏志贺菌的侵袭性和非侵袭性变体在内化后会被变形虫缓慢消化。形态学研究表明，相互作用的特定特征取决于相互作用过程中变形虫膜上甘露糖残基分布的动态变化。
