Lyons G E, Houzelstein D, Sassoon D, Robert B, Buckingham M E
Department of Molecular Biology, U.A. CNRS 1148, Institut Pasteur, Paris, France.
Mol Reprod Dev. 1992 Aug;32(4):303-14. doi: 10.1002/mrd.1080320402.
We report results from a study of Hox-7 expression during mouse embryonic and fetal development and compare the localization of Hox-7 transcripts with those of the retinoic acid receptors. Transcripts were detected by in situ hybridization. Hox-7 expression occurs in (1) cephalic neural crest and its derivatives, (2) sites of ectomesodermal interaction, (3) extraembryonic tissues, and (4) endocardial cells. Hox-7 does not seem to be involved in defining rostrocaudal boundaries, but instead appears to be expressed along the proximodistal axes at these sites. We further investigated the active sites of morphogenesis, which involve an ectomesodermal interaction (e.g., limb buds, visceral arches), including genital tubercle and tail ridge. These are regions highly positive for Hox-7 transcripts, and many are known to be sites for the expression of gamma-retinoic acid receptors (RARs) and cellular retinoic acid binding proteins. Most regions that express Hox-7 are subregions of gamma-RAR expression. In the developing limb bud, expression of Hox-7 takes place in the interdigital region, where it overlaps areas of beta-RAR expression.
我们报告了一项关于小鼠胚胎和胎儿发育过程中Hox - 7表达的研究结果，并将Hox - 7转录本的定位与视黄酸受体的定位进行了比较。通过原位杂交检测转录本。Hox - 7表达出现在：（1）头部神经嵴及其衍生物；（2）外胚层中胚层相互作用部位；（3）胚外组织；（4）心内膜细胞。Hox - 7似乎并不参与确定头尾边界，而是似乎沿着这些部位的近远轴表达。我们进一步研究了形态发生的活跃部位，这些部位涉及外胚层中胚层相互作用（如肢芽、内脏弓），包括生殖结节和尾嵴。这些是Hox - 7转录本高度阳性的区域，并且许多已知是γ - 视黄酸受体（RARs）和细胞视黄酸结合蛋白表达的部位。大多数表达Hox - 7的区域是γ - RAR表达的亚区域。在发育中的肢芽中，Hox - 7在指间区域表达，该区域与β - RAR表达区域重叠。
