Sugita K, Robertson M J, Torimoto Y, Ritz J, Schlossman S F, Morimoto C
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
J Immunol. 1992 Aug 15;149(4):1199-203.
The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti-1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.
大多数静止T淋巴细胞表达CD27抗原，且其似乎在T细胞活化中起关键作用。我们发现一些静止外周血NK细胞也表达CD27。此外，IL-2刺激的NK细胞上CD27表达上调。抗CD27单克隆抗体（抗-1A4）可抑制IL-2活化而非静止的NK细胞的细胞溶解活性。然而，抗-1A4并不影响IL-2活化的NK细胞与肿瘤细胞靶标的结合形成。相反，抗-1A4抑制CD2介导的钙动员及NK细胞颗粒的丝氨酸酯酶活性。这些抑制作用可能部分由抗-1A4诱导的细胞内cAMP水平升高介导。我们的结果表明，CD27抗原在活化NK细胞的调节中起重要作用。
