Rice A, Reiffers J, Bernard P, Fourès C, Bascans E, Lacombe F, Marit G, Broustet A
Unité de Greffe, Hôpital Haut Lévèque, CHR Bordeaux, Pessac, France.
Nouv Rev Fr Hematol (1978). 1992;34(2):167-74.
Long term and semi-solid culture techniques were used to evaluate the quality of stroma produced by bone marrow from 33 normal subjects and 57 patients (46 allogeneic bone marrow and 11 autologous blood stem cell transplant recipients). Bone marrow from transplant recipients was capable of sustained CFU-GM and nucleated cell production in long term culture. However, only 13% of the marrow investigated developed a complete, confluent stromal layer. These stromal abnormalities were observed in spite of complete hematopoietic reconstitution after transplantation and rarely improved with time. Our results suggest that the hematopoietic microenvironment is very fragile and susceptible to long term damage as a result of chemotherapy and the conditioning regimes used prior to transplantation.
采用长期和半固体培养技术，对33名正常受试者和57名患者（46名异基因骨髓移植受者和11名自体造血干细胞移植受者）骨髓产生的基质质量进行评估。移植受者的骨髓在长期培养中能够持续产生集落形成单位-粒细胞巨噬细胞（CFU-GM）和成核细胞。然而，所研究的骨髓中只有13%形成了完整的、融合的基质层。尽管移植后造血功能完全重建，但仍观察到这些基质异常，且很少随时间改善。我们的结果表明，造血微环境非常脆弱，易受化疗和移植前使用的预处理方案的长期损害。
