Hows J M, Marsh J C, Bradley B A, Luft T, Coutinho L, Testa N G, Dexter T M
Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.
Bone Marrow Transplant. 1992;9 Suppl 1:105-8.
In a preliminary study we have shown that optimally collected human umbilical cord (HUC) blood cells grow significantly better in long term cultures (LTC) than normal adult marrow cells (NBM) p = 0.0007. The LTC findings are supported by the observation in clonogenic assay that a similar number of GM-CFC colonies can be grown from HUC blood and NBM mononuclear cells (MNC). Also there is a trend towards a higher proportion of primitive erythroid (BFU-E) and primitive megakaryocyte colonies (MK-CFC containing greater than 20 cells) in HUC blood compared with NBM. We suggest that further work on the feasibility of a HLA typed, cryopreserved HUC blood bank as a source of unrelated haemopoietic 'stem' cells for clinical transplantation is indicated.
在一项初步研究中，我们已表明，最佳采集的人脐带（HUC）血细胞在长期培养（LTC）中的生长情况明显优于正常成人骨髓细胞（NBM），p = 0.0007。集落形成试验观察结果支持了长期培养的发现，即从HUC血和NBM单核细胞（MNC）中可生长出数量相似的GM-CFC集落。此外，与NBM相比，HUC血中原始红系（BFU-E）和原始巨核细胞集落（含20个以上细胞的MK-CFC）的比例有更高的趋势。我们建议，有必要进一步研究建立一个HLA分型、冷冻保存的HUC血库作为临床移植无关造血“干细胞”来源的可行性。
