Selective reduction of glutamate in the rat superior colliculus and dorsal lateral geniculate nucleus after contralateral enucleation.

The effects of afferent lesions on the levels of glutamate, aspartate and gamma-aminobutyric acid (GABA) in the laminae of the superior colliculus (SC) and dorsal lateral geniculate nucleus (dLGN) of the rat were studied, using microassay methods for these amino acids. The analysis was performed 12-14 days after left eye enucleation, or ablation of right visual cortical area, or both left eye enucleation and ablation of right visual cortex. Superficial gray layer (SGL) and deep layers in the SC were dissected out from the thin-sectioned, freeze-dried sample. In the dLGN, the outer and inner laminae were separately dissected. The glutamate contents in the upper half of SGL and outer lamina of dLGN contralateral to eye enucleation decreased significantly (15%). Combination of eye enucleation and visual cortical ablation further decreased the glutamate content in the upper half of the right SGL (29.3%). On the other hand, aspartate and GABA concentrations in the SC and dLGN exhibited no significant reduction after deafferentations. These results indicate that the retino-tectal and retino-geniculate pathway of the rat may be glutamatergic in nature.