Nissen E, Tanneberger S
Allerg Immunol (Leipz). 1976;22(1):69-72.
A method for the detection of cellular tumor-versus-host relations is described, which consist in cultivating human tumor material together with autologous lymphocytes in one organ system and in determinating tumor and lymphocyte DNA synthesis separately before and after mixed organ culture. Advantages and disadvantages of this method are discussed. 58 human tumors were investigated (40 mammary, 13 gastric, 5 bronchial cancers). 37 tumors showed a sufficient in vitro growth. In about 50% of the mixed organ cultures, there was an increase in lymphocyte DNA synthesis compared to the controls. On the other hand, only in 9 cases a decrease in the tumor DNS synthesis could be observed under the influence of autologous lymphocytes. The investigations emphasize the individuality of the tumor-versus-host relations and lead to the consequence of a routine determination of the immune profile by a spectrum of several methods in tumor patients as a prognostic and therapeutic aid.
本文描述了一种检测细胞肿瘤与宿主关系的方法，该方法包括在一个器官系统中共同培养人类肿瘤材料和自体淋巴细胞，并在混合器官培养前后分别测定肿瘤和淋巴细胞的DNA合成。讨论了该方法的优缺点。对58例人类肿瘤进行了研究（40例乳腺癌、13例胃癌、5例支气管癌）。37例肿瘤在体外有足够的生长。在大约50%的混合器官培养中，与对照组相比，淋巴细胞DNA合成增加。另一方面，仅在9例中观察到自体淋巴细胞影响下肿瘤DNA合成减少。这些研究强调了肿瘤与宿主关系的个体性，并导致作为预后和治疗辅助手段，通过多种方法对肿瘤患者的免疫谱进行常规测定的结果。
