Magzoub M M, Stephens H A, Gale E A, Franco Bottazzo G
Faculty of Medicine, University of Gezira, Wad Medani, Sudan.
Scand J Immunol Suppl. 1992;11:187-91. doi: 10.1111/j.1365-3083.1992.tb01649.x.
In this study we report, for the first time, the molecular analysis of HLA-DR and DQ gene frequencies in a large cohort of well-characterized type 1 (insulin-dependent) diabetes mellitus (IDDM) patients (n = 72), and ethnically matched controls (n = 59) collected in sub-Saharan Africa. High molecular mass DNA was prepared and analysed in Southern blots and by oligonucleotide typing. We have shown a strong positive association between IDDM and the Asp 57- DQB1 allele *0201 (DQw2). A rare DR4, DQw2 haplotype was also identified at high frequency in the IDDM cohort. We can now confirm that the association between Asp 57- DQB1 alleles and IDDM, previously reported in ethnically diverse cohorts collected in Western Europe, North America, and South Asia, is also present in an IDDM cohort collected in Africa.
在本研究中，我们首次报告了对撒哈拉以南非洲地区一大群特征明确的1型（胰岛素依赖型）糖尿病（IDDM）患者（n = 72）以及种族匹配的对照人群（n = 59）进行的HLA - DR和DQ基因频率的分子分析。制备了高分子量DNA，并通过Southern印迹法和寡核苷酸分型进行分析。我们发现IDDM与天冬氨酸57 - DQB1等位基因*0201（DQw2）之间存在强正相关。在IDDM队列中还高频鉴定出一种罕见的DR4、DQw2单倍型。我们现在可以确认，先前在西欧、北美和南亚收集的不同种族队列中报道的天冬氨酸57 - DQB1等位基因与IDDM之间的关联，在非洲收集的IDDM队列中也存在。
