人神经母细胞瘤细胞移植，儿茶酚胺能和非儿茶酚胺能：对帕金森大鼠模型旋转行为的影响
Transplantation of human neuroblastoma cells, catecholaminergic and non-catecholaminergic: effects on rotational behavior in Parkinson's rat model.
作者信息
Manaster J S, Feuerman T, Reynolds C P, Markham C H
机构信息
Department of Neurology, University of California, Los Angeles School of Medicine 90024-1769.
出版信息
J Neural Transplant Plast. 1992 Apr-Sep;3(2-3):139-50. doi: 10.1155/NP.1992.139.
Cultured human catecholaminergic and non-catecholaminergic donor cells were used in neural transplantation experiments in a rat model of Parkinson's disease. Using two different human catecholaminergic neuroblastoma cell lines, one control non-catecholaminergic neuroblastoma cell line, and one sham control (tissue culture medium), transplants were made into the striatum using a modified Ungerstedt hemiparkinsonian rat model. Significant decreases in apomorphine-induced rotational behavior were produced by two of three catecholaminergic cell lines. Grafted cells staining positively for tyrosine hydroxylase (TH) and catecholamine fluorescence indicated viable catecholamine activity in the two cell lines which produced reductions in rotational behavior. Catecholamine fluorescence was not detected in either of the two controls. These data suggest a link between catecholamine secretion by transplanted cells and motor improvement using a rat rotational behavior model.
在帕金森病大鼠模型的神经移植实验中，使用了培养的人儿茶酚胺能和非儿茶酚胺能供体细胞。利用两种不同的人儿茶酚胺能神经母细胞瘤细胞系、一种对照非儿茶酚胺能神经母细胞瘤细胞系和一种假手术对照（组织培养基），采用改良的Ungerstedt偏侧帕金森病大鼠模型将移植体植入纹状体。三种儿茶酚胺能细胞系中的两种使阿扑吗啡诱导的旋转行为显著减少。对酪氨酸羟化酶（TH）和儿茶酚胺荧光呈阳性染色的移植细胞表明，在这两种导致旋转行为减少的细胞系中存在有活力的儿茶酚胺活性。在两个对照组中均未检测到儿茶酚胺荧光。这些数据表明，使用大鼠旋转行为模型，移植细胞分泌儿茶酚胺与运动改善之间存在联系。
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