Cameron J L, Pomerantz S M, Layden L M, Amico J A
Department of Behavioral Neuroscience, University of Pittsburgh School of Medicine, Pennsylvania 15261.
J Clin Endocrinol Metab. 1992 Sep;75(3):855-60. doi: 10.1210/jcem.75.3.1355489.
Administration of the dopamine receptor agonist apomorphine causes a dose-dependent increase in plasma oxytocin concentrations and dose-specific behavioral changes in rodents. To investigate whether dopamine receptor agonists will elicit similar neuroendocrine and behavioral effects in primates, we administered graded doses of apomorphine and the respective dopamine D1 and D2 receptor agonists, CY 208-243 and LY 163502, to monkeys and monitored plasma concentrations of oxytocin and behavior. Five female rhesus, two male rhesus, and two male cynomolgus monkeys had chronic indwelling venous catheters implanted and were maintained on standard jacket/tether/swivel systems to allow remote blood sample collection. During experiments, blood samples were collected 10 and 5 min before drug injection and at 2- to 120-min intervals after each injection. Apomorphine (50-400 micrograms/kg) and LY 163502 (10-100 micrograms/kg) elicited dose-dependent stimulations of oxytocin secretion. CY 208-243 (100-400 micrograms/kg) did not significantly affect oxytocin secretion. Low doses of apomorphine (50-100 micrograms/kg) and LY 163502 (10-25 micrograms/kg) elicited yawning, and high doses of apomorphine (200-400 micrograms/kg) and LY 163502 (50-100 micrograms/kg) elicited stereotypic behaviors. No behavioral effects of CY 208-243 (100-400 micrograms/kg) were observed. The magnitude of the oxytocin secretory responses varied among animals, but was similar in male and female monkeys. In summary, apomorphine and LY 163502 both elicited dose-related stimulation of oxytocin secretion coupled with dose-specific behavioral changes in male and female monkeys, while no effects of CY 208-243 on these parameters were observed. We conclude that dopamine receptor agonists, and in particular D2 agonists, may be useful tools for studies exploring the physiological and behavioral actions of oxytocin in primates.
给予多巴胺受体激动剂阿扑吗啡会使啮齿动物的血浆催产素浓度呈剂量依赖性增加，并引起特定剂量的行为变化。为了研究多巴胺受体激动剂在灵长类动物中是否会引发类似的神经内分泌和行为效应，我们给猴子注射了不同剂量的阿扑吗啡以及各自的多巴胺D1和D2受体激动剂CY 208 - 243和LY 163502，并监测了催产素的血浆浓度和行为。五只雌性恒河猴、两只雄性恒河猴和两只雄性食蟹猴植入了慢性留置静脉导管，并通过标准的夹克/系绳/旋转系统进行饲养，以便远程采集血样。在实验过程中，在药物注射前10分钟和5分钟以及每次注射后每隔2至120分钟采集血样。阿扑吗啡（50 - 400微克/千克）和LY 163502（10 - 100微克/千克）引起了催产素分泌的剂量依赖性刺激。CY 208 - 243（100 - 400微克/千克）对催产素分泌没有显著影响。低剂量的阿扑吗啡（50 - 100微克/千克）和LY 163502（10 - 25微克/千克）引起打哈欠，高剂量的阿扑吗啡（200 - 400微克/千克）和LY 163502（50 - 100微克/千克）引起刻板行为。未观察到CY 208 - 243（100 - 400微克/千克）的行为效应。催产素分泌反应的幅度在不同动物之间有所不同，但在雄性和雌性猴子中相似。总之，阿扑吗啡和LY 163502均引起了雄性和雌性猴子催产素分泌的剂量相关刺激以及特定剂量的行为变化，而未观察到CY 208 - 243对这些参数的影响。我们得出结论，多巴胺受体激动剂，尤其是D2激动剂，可能是探索催产素在灵长类动物中的生理和行为作用的有用工具。
