• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Differentiation of mouse embryonic stem cells to lympho-hematopoietic lineages in vitro.小鼠胚胎干细胞在体外向淋巴造血谱系的分化
Dev Immunol. 1992;2(1):29-50. doi: 10.1155/1992/79518.
2
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?CD123高表达浆细胞样前树突状细胞：正在经历细胞命运转变的祖细胞？
J Immunol. 2002 Jul 1;169(1):75-83. doi: 10.4049/jimmunol.169.1.75.
3
Development of mouse embryonic stem (ES) cells: IV. Differentiation to mature T and B lymphocytes after implantation of embryoid bodies into nude mice.小鼠胚胎干细胞（ES细胞）的发育：IV. 将胚状体植入裸鼠后分化为成熟的T和B淋巴细胞。
Dev Immunol. 1995;4(2):79-84. doi: 10.1155/1995/52962.
4
Development of rat CD45+ 13-day-old fetal liver cells in SCID mouse fetal thymic organ cultures.
Int Immunol. 1999 Jul;11(7):1119-29. doi: 10.1093/intimm/11.7.1119.
5
Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation.具有不同T细胞受体的两种肠道上皮内CD8 +淋巴细胞群体：肠道上皮在T细胞分化中的作用。
J Exp Med. 1991 Feb 1;173(2):471-81. doi: 10.1084/jem.173.2.471.
6
DNFB contact sensitivity (CS) in BALB/c and C3H/He mice: requirement for early-occurring, early-acting, antigen-specific, CS-initiating cells with an unusual phenotype (Thy-1+, CD5+, CD3-, CD4-, CD8-, sIg-, B220+, MHC class II-, CD23+, IL-2R-, IL-3R+, Mel-14-, Pgp-1+, J11d+, MAC-1+, LFA-1+, and Fc gamma RII+).BALB/c和C3H/He小鼠中的二硝基氟苯接触敏感性（CS）：对具有异常表型（Thy-1 +、CD5 +、CD3 -、CD4 -、CD8 -、sIg -、B220 +、MHC II类 -、CD23 +、IL - 2R -、IL - 3R +、Mel - 14 -、Pgp - 1 +、J11d +、MAC - 1 +、LFA - 1 +和FcγRII +）的早期出现、早期起作用、抗原特异性、CS起始细胞的需求。
J Invest Dermatol. 1994 Mar;102(3):321-7. doi: 10.1111/1523-1747.ep12371790.
7
Establishment and characterization of lymphoid and myeloid mixed-cell populations from mouse late embryoid bodies, "embryonic-stem-cell fetuses".从小鼠晚期类胚体（“胚胎干细胞胎儿”）建立淋巴样和髓样混合细胞群体并进行表征。
Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2541-5. doi: 10.1073/pnas.89.7.2541.
8
The fetal liver counterpart of adult common lymphoid progenitors gives rise to all lymphoid lineages, CD45+CD4+CD3- cells, as well as macrophages.成体常见淋巴样祖细胞在胎儿肝脏中的对应细胞可分化产生所有淋巴谱系、CD45+CD4+CD3-细胞以及巨噬细胞。
J Immunol. 2001 Jun 1;166(11):6593-601. doi: 10.4049/jimmunol.166.11.6593.
9
CD3 delta and epsilon gene expression in CD3-CD16+ natural killer cell clones derived from thymic precursors.源自胸腺前体细胞的CD3-CD16+自然杀伤细胞克隆中CD3δ和ε基因的表达
Hum Immunol. 1995 Aug;43(4):283-94. doi: 10.1016/0198-8859(95)91034-u.
10
The kinetics of T cell antigen receptor expression by subgroups of CD4+8+ thymocytes: delineation of CD4+8+3(2+) thymocytes as post-selection intermediates leading to mature T cells.CD4+8+胸腺细胞亚群T细胞抗原受体表达的动力学：将CD4+8+3(2+)胸腺细胞描绘为通向成熟T细胞的选择后中间体。
J Exp Med. 1991 Feb 1;173(2):323-32. doi: 10.1084/jem.173.2.323.

引用本文的文献

1
Derivation of es Cells From Early Stage Preimplantation Embryos and Characterisation of their Cardiac Differentiation Potential in Mice.从植入前早期胚胎中衍生胚胎干细胞及其在小鼠中的心脏分化潜能表征
J Stem Cells Regen Med. 2012 Apr 14;8(1):12-20. doi: 10.46582/jsrm.0801003. eCollection 2012.
2
Effects of Growth Factors on Establishment and Propagation of Embryonic Stem Cells from Very Early Stage IVF Embryos and Their Characterization in Buffalo.生长因子对牛早期体外受精胚胎胚胎干细胞建立和增殖的影响及其特性。
Int J Stem Cells. 2012 Nov;5(2):96-103. doi: 10.15283/ijsc.2012.5.2.96.

小鼠胚胎干细胞在体外向淋巴造血谱系的分化

Differentiation of mouse embryonic stem cells to lympho-hematopoietic lineages in vitro.

作者信息

Chen U

机构信息

Basel Institute for Immunology, Switzerland.

出版信息

Dev Immunol. 1992;2(1):29-50. doi: 10.1155/1992/79518.

DOI:10.1155/1992/79518
PMID:1355679
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2275843/
Abstract

Mouse embryonic stem (ES) cells can differentiate in culture to late stages of many cell lineages. I have found culture conditions that are favorable for development in vitro of ES cells into hematopoietic cells at a stage equivalent to day 11-14 of fetal liver development. I describe here: (1) the growth conditions necessary for maintenance of ES cells in an undifferentiated state, and the conditions that allow differentiation of cystic embryoid bodies that contain precursors of most hematopoietic cell lineages, including lymphoid cells; (2) the development of lymphoid vessels from ES fetuses in vivo; (3) the characterization of lymphoid, erythroid, megakaryoid, and myeloid cells from ES fetuses; and (4) the cloning of cell lines representing lymphoid, myeloid lineage cells from differentiated ES cells.

摘要

小鼠胚胎干细胞（ES细胞）在培养中可分化至许多细胞谱系的后期阶段。我已发现有利于ES细胞在体外发育为造血细胞的培养条件，该阶段相当于胎儿肝脏发育的第11 - 14天。我在此描述：（1）维持ES细胞处于未分化状态所需的生长条件，以及允许含有大多数造血细胞谱系（包括淋巴细胞）前体的囊性胚状体分化的条件；（2）ES胎儿在体内淋巴血管的发育；（3）ES胎儿来源的淋巴细胞、红细胞、巨核细胞和髓细胞的特征；以及（4）从分化的ES细胞中克隆代表淋巴细胞、髓细胞谱系细胞的细胞系。