Salazar M, Yunis I, Alosco S M, Chopek M, Yunis E J
Dana Farber Cancer Institute, Boston, Mass.
Tissue Antigens. 1992 Apr;39(4):203-8. doi: 10.1111/j.1399-0039.1992.tb01936.x.
We have used a PCR-RFLP method with one generic amplification of HLA-DPB1 second exon and 6 endonucleases to differentiate the 19 HLA-DPB1 alleles and 171 heterozygous combinations. The set of primers used in our studies produced fragment sizes different from those published before (1). The HLA-DPB1 alleles in Caucasians showed a higher frequency of DPB10401 and DPB10402, when compared to a small group of Colombians who showed a higher frequency of DPB10402 and DPB10201. We found three HLA-DPB1 alleles associated with two HLA haplotypes that result from non-random association of alleles: DPB10401 with HLA-A26, B38, DR4, DQA10301 and DPB10101 and DPB10401 with HLA-A1, B8, DR3, DQA1*0501. We also report that 70% of combinations between HLA (generic A,B,C,DR) and DQA1-identical MLC-unreactive cell mixtures showed HLA-DPB1 mismatches, suggesting that HLA-DPB1 differences are not important in MLC reactivity.
我们采用了一种PCR-RFLP方法，通过对HLA-DPB1第二外显子进行一次通用扩增，并使用6种核酸内切酶来区分19种HLA-DPB1等位基因和171种杂合组合。我们研究中使用的引物组产生的片段大小与之前发表的不同（1）。与一小群哥伦比亚人相比，高加索人中HLA-DPB1等位基因显示DPB10401和DPB10402的频率更高，而哥伦比亚人显示DPB10402和DPB10201的频率更高。我们发现三个HLA-DPB1等位基因与两种HLA单倍型相关，这两种单倍型是由等位基因的非随机关联产生的：DPB10401与HLA-A26、B38、DR4、DQA10301相关，DPB10401与HLA-A1、B8、DR3、DQA10501相关。我们还报告说，HLA（通用A、B、C、DR）和DQA1相同的MLC无反应性细胞混合物之间70%的组合显示HLA-DPB1不匹配，这表明HLA-DPB1差异在MLC反应性中并不重要。
