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软骨研究:正常兔耳软骨的电子显微镜观察

Studies on cartilage: electron microscope observations on normal rabbit ear cartilage.

作者信息

SHELDON H, ROBINSON R A

出版信息

J Biophys Biochem Cytol. 1958 Jul 25;4(4):401-6. doi: 10.1083/jcb.4.4.401.

Abstract

Normal rabbit ear cartilage studied with the light and electron microscope shows chondrocytes in which large lipide spherules, and an abundance of glycogen, a few small mitochondria, and relatively few elements of the endoplasmic reticulum can be identified. The chondrocytes contain, in addition, a material which stains strongly with acid fuchsin and appears in the electron microscope as a relatively dense felt-work. In electron micrographs, the matrix of normal rabbit ear cartilage consists of two components: a uniformly distributed moderately dense substance which appears as a fine meshwork without any particular pattern extending from cartilage cell border to cartilage cell border; and a three-dimensional anastomotic network of more dense material, which is best described as "felt-like" lying between the cells. The similarity between the felt-like material of the matrix and the elastic fibers described in previous electron microscope observations is discussed.

摘要

用光学显微镜和电子显微镜研究正常兔耳软骨时发现,软骨细胞内有大的脂质球、大量糖原、少量小线粒体以及相对较少的内质网成分。此外,软骨细胞还含有一种物质,它能被酸性品红强烈染色,在电子显微镜下呈现为相对致密的网络状。在电子显微镜照片中,正常兔耳软骨的基质由两部分组成:一种均匀分布的中等密度物质,呈现为从软骨细胞边界延伸到软骨细胞边界的无特定图案的精细网络;以及一种更致密物质的三维吻合网络,最好描述为位于细胞之间的“毡状”。文中还讨论了基质中毡状物质与先前电子显微镜观察中描述的弹性纤维之间的相似性。

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