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激肽释放酶、胰蛋白酶和淀粉酶在犬胰腺中的细胞内定位。

The intracellular localization of kallikrein, trypsin and amylase in dog pancreas.

作者信息

Bhoola K D, Dorey G

出版信息

J Physiol. 1971 May;214(3):553-70. doi: 10.1113/jphysiol.1971.sp009448.

DOI:10.1113/jphysiol.1971.sp009448
PMID:5580865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1331854/
Abstract
  1. Subcellular organelles containing kallikrein (-ogen), trypsinogen and amylase have been isolated from homogenates of dog pancreas and found on discontinuous sucrose density-gradients to equilibrate with 1.7-1.775 M sucrose.2. The electron microscopic appearance of the isolated enzyme-containing organelles appeared to be identical with that of the secretory (zymogen) granules seen in situ in the acinar cells of the pancreas.3. The sequence of events associated with the storage and intracellular transport of pancreatic secretory enzymes is reviewed. The results obtained in this study have been used to delineate the probable intracellular pathway taken by kallikrein, trypsin and amylase from synthesis to secretion.4. The physiological role of the glandular kallikreins is discussed.
摘要

  1. 已从犬胰腺匀浆中分离出含有激肽释放酶（原）、胰蛋白酶原和淀粉酶的亚细胞细胞器，并发现其在不连续蔗糖密度梯度上与1.7 - 1.775M蔗糖达到平衡。

  2. 分离出的含酶细胞器的电子显微镜外观似乎与胰腺腺泡细胞原位所见的分泌（酶原）颗粒相同。

  3. 综述了与胰腺分泌酶的储存和细胞内运输相关的一系列事件。本研究获得的结果已用于描绘激肽释放酶、胰蛋白酶和淀粉酶从合成到分泌可能的细胞内途径。

  4. 讨论了腺体激肽释放酶的生理作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/4bdd60b7a3b2/jphysiol01029-0206-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/604c716980eb/jphysiol01029-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/700d758a77b1/jphysiol01029-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/2f26af76e5bc/jphysiol01029-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/5451ab13314a/jphysiol01029-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/08757660e613/jphysiol01029-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/6c5c393dbf82/jphysiol01029-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/e8f47a25267c/jphysiol01029-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/4bdd60b7a3b2/jphysiol01029-0206-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/604c716980eb/jphysiol01029-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/700d758a77b1/jphysiol01029-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/2f26af76e5bc/jphysiol01029-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/5451ab13314a/jphysiol01029-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/08757660e613/jphysiol01029-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/6c5c393dbf82/jphysiol01029-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/e8f47a25267c/jphysiol01029-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c2/1331854/4bdd60b7a3b2/jphysiol01029-0206-b.jpg

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引用本文的文献

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Tissue kallikrein synthesis and its modification by testosterone or low dietary sodium.组织激肽释放酶的合成及其受睾酮或低钠饮食的影响。
Biochem J. 1984 Feb 15;218(1):37-43. doi: 10.1042/bj2180037.
2
The metabolism of cyclic nucleotides in the guinea-pig pancreas. Cyclic AMP phosphodiesterase and cyclic GMP phosphodiesterase.豚鼠胰腺中环状核苷酸的代谢。环磷酸腺苷磷酸二酯酶和环磷酸鸟苷磷酸二酯酶。
Biochem J. 1980 Feb 15;186(2):491-8. doi: 10.1042/bj1860491.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The mechanism of enzyme secretion by the cell. I. Storage of amylase in the zymogen granules of the rat-parotis gland.细胞分泌酶的机制。I. 淀粉酶在大鼠腮腺腺泡细胞酶原颗粒中的储存。
Biochim Biophys Acta. 1961 Jun 10;50:102-12. doi: 10.1016/0006-3002(61)91065-4.
3
The formation of pancreatic juice proteins studies with labelled amino acids.用标记氨基酸对胰液蛋白质形成的研究。
Acta Physiol Scand Suppl. 1959;46(161):1-99.
4
Studies on cartilage: electron microscope observations on normal rabbit ear cartilage.软骨研究：正常兔耳软骨的电子显微镜观察
J Biophys Biochem Cytol. 1958 Jul 25;4(4):401-6. doi: 10.1083/jcb.4.4.401.
5
A cytochemical study on the pancreas of the guinea pig. I. Isolation and enzymatic activities of cell fractions.豚鼠胰腺的细胞化学研究。I. 细胞组分的分离及酶活性
J Biophys Biochem Cytol. 1958 Mar 25;4(2):203-18. doi: 10.1083/jcb.4.2.203.
6
Isolation of the zymogen granules of dog pancreas and a study of their properties.犬胰腺酶原颗粒的分离及其性质研究。
Biochim Biophys Acta. 1955 Nov;18(3):379-88. doi: 10.1016/0006-3002(55)90101-3.
7
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
8
[Intracellular distribution of Kallikrein and bradykininogen in hog pancreas].[猪胰腺中激肽释放酶和缓激肽原的细胞内分布]
Biochem Z. 1955;326(6):420-3.
9
Distributions of chymotrypsinogen, procarboxypeptidase, desoxyribonuclease, and ribonuclease in bovine pancreas.胰凝乳蛋白酶原、羧肽酶原、脱氧核糖核酸酶和核糖核酸酶在牛胰腺中的分布。
Exp Cell Res. 1954 Feb;6(1):240-2. doi: 10.1016/0014-4827(54)90167-6.
10
The subcellular localization of kallikrein, amylase and acetylcholine in the submaxillary gland of the guinea-pig.豚鼠颌下腺中激肽释放酶、淀粉酶和乙酰胆碱的亚细胞定位。
J Physiol. 1966 Jun;184(3):663-72. doi: 10.1113/jphysiol.1966.sp007939.