Kibue M A, Ehara M, Waiyaki P G
Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi.
East Afr Med J. 1992 Aug;69(8):442-4.
For Vibrio cholerae 01 to overcome the normal intestinal clearing mechanisms and facilitate colonization in the host gut, fine filamentous structures covering the surface of all the cell must exist. These fimbriae-like structures were investigated in this study. Selection of the K23 V. cholerae 01 biotype el-tor isolated in Kisumu, Kenya, was based on its proteinase activity and ability to agglutinate 0.2M ammonium sulphate. This strain was cultured in modified tryptone broth pH 8.4, at 37 degrees C overnight. Electron microscopy of the strain revealed the presence of fimbriae-like structures which varied in number. Some cells showed more than 200, whereas in other cells only several filamentous structures were detected. It is suggested that these structures could be a possible candidate for a cholera vaccine.
为使霍乱弧菌O1型克服正常的肠道清除机制并促进在宿主肠道内的定殖，必须存在覆盖所有细胞表面的精细丝状结构。本研究对这些菌毛样结构进行了调查。选择在肯尼亚基苏木分离的K23霍乱弧菌O1型埃尔托生物型，是基于其蛋白酶活性和凝集0.2M硫酸铵的能力。该菌株在pH 8.4的改良胰蛋白胨肉汤中于37℃培养过夜。对该菌株进行电子显微镜检查发现存在数量各异的菌毛样结构。一些细胞显示有200多个，而在其他细胞中仅检测到几根丝状结构。有人提出这些结构可能是霍乱疫苗的一个候选对象。
