脑室内注射氯化锂对大鼠中基底下丘脑单胺浓度的影响。

Effects of i.c.v. lithium chloride administration on monoamine concentration in rat mediobasal hypothalamus.

作者信息

Otero Losada M E, Rubio M C

机构信息

Instituto de Investigaciones Farmacológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (ININFA) (CONICET), Buenos Aires, Argentina.

出版信息

Eur J Pharmacol. 1992 May 14;215(2-3):185-9. doi: 10.1016/0014-2999(92)90027-2.

DOI:10.1016/0014-2999(92)90027-2

PMID:1356789

Abstract

We investigated the acute effects of a single i.c.v. injection of lithium chloride (LiCl) the neuroamine content of the rat mediobasal hypothalamus (MBH). The effects of lithium on amine synthesis and degradation enzymes were also studied in vitro. Noradrenaline (NA), dopamine (DA), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) concentrations were reduced 10 min after i.c.v. injection of 24 nmol of LiCl and returned to control values 30 min after the injection. Two nmol of LiCl reduced the concentration of DA (10 and 30 min after injection) and 5-HIAA (30 min after injection). LiCl (0.5-10 mM) inhibited tyrosine hydroxylase activity (catecholamine synthesis) in vitro in a concentration dependent manner. The i.c.v. administration of a high dose of LiCl reduced the content of neuroamines in the MBH. This might result from and inhibition of synthesis. A possible link between the observed changes and some reported side effects of lithium therapy is discussed.

摘要

我们研究了单次脑室内注射氯化锂（LiCl）对大鼠中基底下丘脑（MBH）神经胺含量的急性影响。还在体外研究了锂对胺合成和降解酶的影响。脑室内注射24 nmol LiCl后10分钟，去甲肾上腺素（NA）、多巴胺（DA）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）浓度降低，注射后30分钟恢复至对照值。2 nmol LiCl降低了DA浓度（注射后10分钟和30分钟）和5-HIAA浓度（注射后30分钟）。LiCl（0.5 - 10 mM）在体外以浓度依赖性方式抑制酪氨酸羟化酶活性（儿茶酚胺合成）。高剂量LiCl脑室内给药降低了MBH中神经胺的含量。这可能是由于合成受到抑制所致。文中讨论了观察到的这些变化与锂疗法某些已报道的副作用之间可能存在的联系。