Engers H D, MacDonald H R, Cerottini J C, Brunner K T
Eur J Immunol. 1975 Mar;5(3):223-5. doi: 10.1002/eji.1830050316.
The generation of mouse cytolytic T lymphocytes (CTL) in primary or secondary mixed leukocyte cultures (MLC) is greatly enhanced by the addition of 2-mercaptoethanol (2-ME) to the culture medium. This enhancement can be equally demonstrated with either reduced or oxidized 2-ME. Addition of 2-ME to MLC as late as 3 days after the initiation of the culture results in a peak CTL response which is nearly as high as the one in cultures containing 2-ME throughout the 4 day-incubation period. Quantitative analysis of the CTL response in MLC supplemented with 2-ME on day 3 shows a 50-fold increase in lytic activity within 24 h, suggesting that cell differentiation, in addition to proliferation may be affected by this agent.
在原代或二代混合淋巴细胞培养物（MLC）中，向培养基中添加2-巯基乙醇（2-ME）可显著增强小鼠细胞溶解性T淋巴细胞（CTL）的生成。无论是还原型还是氧化型2-ME，均可同等程度地表现出这种增强作用。在培养开始后长达3天再向MLC中添加2-ME，会产生一个峰值CTL反应，该反应几乎与在整个4天培养期都含有2-ME的培养物中的反应一样高。对第3天添加2-ME的MLC中的CTL反应进行定量分析显示，24小时内裂解活性增加了50倍，这表明除了增殖外，细胞分化也可能受该试剂影响。
