Talmud P J, Boerwinkle E, Xu C F, Tikkanen M J, Pietinen P, Huttunen J K, Humphries S
Charing Cross Sunley Research Centre, London, UK.
Genet Epidemiol. 1992;9(4):249-60. doi: 10.1002/gepi.1370090404.
We have examined whether variation at the apolipoprotein (apo) B, apo E, apo AII, and apo AI-CIII-AIV genes affected the relationship between dietary intake and serum lipid traits in individuals who had participated in dietary intervention from a basal high fat diet to a low fat diet followed by a return to their natural diet, the switchback. On both the basal and switchback diets where the variance of dietary intake was great, there was a significant correlation between P/S ratio and serum total, low-density lipoprotein (LDL) cholesterol, and apo AI levels. In addition dietary cholesterol (dchol) levels correlated significantly with serum apo AI levels on the basal diet. Comparing the difference between basal and intervention (delta 1) and between switchback and intervention diets (delta 2), changes in dchol and P/S ratio correlated significantly with changes in serum total, high-density lipoprotein (HDL) and LDL cholesterol, and apo B levels. There was a significant correlation between monounsaturated fatty acid (MUFA) and apo AI levels during both changes. Furthermore we have examined whether the relationship between variables was homogeneous among genotypes of candidate gene polymorphisms. A heterogeneous effect (P less than 0.01) was seen among genotypes of the PvuII-AIV restriction fragment length polymorphism (RFLP) on the correlation of serum LDL cholesterol levels and dietary MUFA during both dietary changes (delta 1 and delta 2). A heterogeneous effect among genotypes of the apo B XbaI RFLP on the correlation between dchol versus total and LDL cholesterol during the change delta 1, but not delta 2, was observed. Thus our results show that both dietary components and genetic variation affect the response of serum lipid, lipoprotein, and apolipoprotein levels to dietary change.
我们研究了载脂蛋白（apo）B、apo E、apo AII和apo AI-CIII-AIV基因的变异是否影响了参与从基础高脂肪饮食改为低脂饮食然后恢复自然饮食（折返饮食）的个体的饮食摄入与血脂特征之间的关系。在基础饮食和折返饮食中，饮食摄入的差异很大，多不饱和/饱和脂肪酸（P/S）比值与血清总胆固醇、低密度脂蛋白（LDL）胆固醇和apo AI水平之间存在显著相关性。此外，基础饮食中饮食胆固醇（dchol）水平与血清apo AI水平显著相关。比较基础饮食与干预饮食之间的差异（δ1）以及折返饮食与干预饮食之间的差异（δ2），dchol和P/S比值的变化与血清总胆固醇、高密度脂蛋白（HDL）和LDL胆固醇以及apo B水平的变化显著相关。在两种变化过程中，单不饱和脂肪酸（MUFA）与apo AI水平之间存在显著相关性。此外，我们还研究了候选基因多态性的基因型之间变量关系是否同质。在两种饮食变化（δ1和δ2）期间，PvuII-AIV限制性片段长度多态性（RFLP）的基因型在血清LDL胆固醇水平与饮食MUFA的相关性方面存在异质性效应（P<0.01）。在变化δ1期间观察到apo B XbaI RFLP的基因型在dchol与总胆固醇和LDL胆固醇之间的相关性方面存在异质性效应，但在δ2期间未观察到。因此，我们的结果表明，饮食成分和基因变异均会影响血清脂质、脂蛋白和载脂蛋白水平对饮食变化的反应。
