Röllinghoff M, Pfizenmeier K, Trostmann H, Wagner H
Eur J Immunol. 1975 Aug;5(8):560-4. doi: 10.1002/eji.1830050811.
It was tested whether T lymphocytes, when stimulated in vitro by M locus-coded lymphocyte activating determinants (LAD), are able to mediate cytotoxic effector functions. The assay for cytotoxicity included both the use of purified appropriate target cells (i.e. purified lipopolysaccharide blasts) as well as the use of phytohemagglutinin dependent cytolysis as a model for detecting cytotoxic T lymphocytes (CTL). Although strong proliferative responses were obtained in the mixed lymphocyte culture, the T cell blast generated did not display any detectable cytotoxic effector function. Thus, it is concluded that LAD, at least in the M locus-dependent system, do have the capacity to induce T cell proliferation but do not induce CTL.
测试了T淋巴细胞在体外受到M位点编码的淋巴细胞激活决定簇（LAD）刺激时，是否能够介导细胞毒性效应功能。细胞毒性测定方法既包括使用纯化的合适靶细胞（即纯化的脂多糖母细胞），也包括使用植物血凝素依赖性细胞溶解作为检测细胞毒性T淋巴细胞（CTL）的模型。尽管在混合淋巴细胞培养中获得了强烈的增殖反应，但产生的T细胞母细胞未显示出任何可检测到的细胞毒性效应功能。因此，得出结论，至少在M位点依赖性系统中，LAD确实有能力诱导T细胞增殖，但不能诱导CTL。
